Abstract |
A highly specific and sensitive radioimmunoassay (RIA) has been established for determination of endothelin-3 like immunoreactivity in human plasma to investigate its possible role in hemodynamic alterations due to liver disease. Crossreactivity with other endothelin isoforms was always below 4 %, the lower detection limit following extraction on Sep-Pak C18 cartridges was 0.5 pg/ml. The concentration of endothelin-3 (mean +/- SEM) was 4.16 +/- 0.56 pg/ml (n = 13) in plasma of patients with cirrhosis of the liver, three fold higher than in age matched controls (1.35 +/- 0.27 pg/ml, n = 12, p less than 0.01). Plasma immunoreactivity was confirmed to be endothelin-3 related by reverse-phase HPLC. These data could suggest a role of plasma endothelin-3 in circulatory changes, as they occur in cirrhosis of the liver.
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Authors | V Gülberg, A L Gerbes, A M Vollmar, G Paumgartner |
Journal | Life sciences
(Life Sci)
Vol. 51
Issue 14
Pg. 1165-9
( 1992)
ISSN: 0024-3205 [Print] Netherlands |
PMID | 1518378
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Chromatography, High Pressure Liquid
- Endothelins
(blood)
- Humans
- Liver Cirrhosis
(blood)
- Middle Aged
- Radioimmunoassay
- Reproducibility of Results
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