Radiolabeling, biodistribution, and dosimetry of (123)I-mAb 14C5: a new mAb for radioimmunodetection of tumor growth and metastasis in vivo.

Abstract

UNLABELLED:

This study reports on the in vitro evaluation, biodistribution, and dosimetry of (123)I-labeled monoclonal antibody (mAb) 14C5, a new antibody-based agent proposed for radioimmunodetection of tumor growth and metastasis in vivo.

METHODS:

(123)I-mAb 14C5 was prepared by direct iodination and tested for stability in vitro. Binding assays were performed on human SK-BR-3 and HeLa carcinoma cells to investigate the antigen expression, antibody affinity, and kinetics of tracer binding. For the biodistribution and dosimetry study, 3- to 4-wk-old NMRI mice were injected intravenously with (123)I-mAb 14C5 (148.0 +/- 7.4 kBq per mouse) and killed at preset time intervals. Organs, blood, urine, and feces were counted for radioactivity uptake, and the data were expressed as the percentage injected dose per gram tissue (%ID/g tissue) or %ID. The MIRDOSE3.0 program was applied to extrapolate the estimated absorbed radiation doses for various organs to the human reference adult.

RESULTS:

(123)I-mAb 14C5 was obtained in radiochemical yields of 85.0% +/- 2.5% and radiochemical purities were >97%. The iodinated antibody demonstrated good in vitro stability with 93.6% +/- 0.1% of (123)I-mAb 14C5 remaining intact at 24 h after radiolabeling. (123)I-mAb 14C5 bound to SK-BR-3 cells (dissociation constant [K(d)] approximately 0.85 +/- 0.17 nmol/L) and HeLa cells (K(d) approximately 1.71 +/- 0.17 nmol/L) with nanomolar affinity and high specificity, whereas both cell types exhibited a high CA14C5 antigen expression (maximum number of binding sites [B(max)] = 40.6 +/- 5.2 and 57.1 +/- 9.6 pmol/L, respectively). In mice, (123)I-mAb 14C5 accumulated primarily in lungs (20.4 %ID/g), liver (15.1 %ID/g), and kidneys (11.1 %ID/g) within 5 min after injection. A delayed uptake was observed in stomach (12.8 %ID/g) and urinary bladder (8.7 %ID/g) at 3 and 6 h, respectively, after injection. Radioactivity clearance was predominantly urinary, with 44.9 +/- 4.5 %ID excreted during the initial 48 h after administration (cumulative amount). The highest absorbed radiation doses determined for the human reference adult were received by the urinary bladder wall (0.1200-0.1210 mGy/MBq), liver (0.0137-0.0274 mGy/MBq), uterus (0.0196-0.0207 mGy/MBq), and lower large intestine wall (0.0139-0.0258 mGy/MBq). The average effective dose resulting from a single (123)I-mAb 14C5 injection was estimated to be 0.017-0.022 mSv/MBq.

CONCLUSION:

(123)I-mAb 14C5 shows good in vitro biologic activity and favorable biodistribution properties for imaging carcinomas of different origin and provides an acceptable radiation dose to the patient.

• Authors: Christophe M M Lahorte, Klaus Bacher, Ingrid Burvenich, Elisabeth D Coene, Claude Cuvelier, Christian De Potter, Hubert Thierens, Christophe Van de Wiele, Rudi A Dierckx, Guido Slegers
• Journal: Journal of nuclear medicine : official publication, Society of Nuclear Medicine (J Nucl Med) Vol. 45 Issue 6 Pg. 1065-73 (Jun 2004) ISSN: 0161-5505 [Print] United States
• PMID: 15181142 (Publication Type: Comparative Study, Journal Article)

Chemical References

• 14C5 monoclonal antibody
• Antibodies, Monoclonal
• Radiopharmaceuticals

Topics

• Animals
• Antibodies, Monoclonal (analysis, chemistry, pharmacokinetics)
• Body Burden
• Carcinoma (blood, diagnostic imaging, metabolism, urine)
• Cell Line, Tumor (diagnostic imaging, metabolism)
• Feces (chemistry)
• Female
• HeLa Cells
• Humans
• Isotope Labeling (methods)
• Metabolic Clearance Rate
• Mice
• Neoplasms (blood, diagnostic imaging, metabolism, urine)
• Organ Specificity
• Radioimmunodetection (methods)
• Radiometry (methods)
• Radiopharmaceuticals (analysis, chemical synthesis, pharmacokinetics)
• Tissue Distribution
• Whole-Body Counting