Effect of crocetin on cardiac hypertrophy induced by overloading pressure in rats.

Abstract

AIM:

To study the influence of crocetin on cardiac hypertrophy induced by overloading pressure in rats.

METHODS:

The model of cardiac hypertrophy was produced by overloading pressure in rats. The animals were divided into five groups: sham-operation group (0.5% CMC-Na, ig), model group (operation + 0.5% CMC-Na, ig), captopril group (operation + 50 mg x kg(-1), ig), crocetin I (100 mg x kg(-1), ig) and crocetin II (50 mg x kg(-1), ig). All animals were treated for 30 d by ig. Then, cardiac indexes were examined. The activity of ATPase and the hydroxyproline content in heart were assayed by colorimetric analysis. Matrix metalloproteinases (MMPs) activity was assayed by SDS-PAGE zymography.

RESULTS:

Compared with the model group, crocetin was found to significantly reduce the cardiac indexes and the content of hydroxyproline in heart, increase the activity of Na+ , K+ -ATPase, Ca2+, Mg2+ -ATPase and inhibit MMPs activity.

CONCLUSION:

The activity of MMPs may play a key role in the cardiac hypertrophy induced by overloading pressure, and proprably as a result of decreasing the activity of MMPs. Crocetin was shown to prevent remodeling of cardiac hypertrophy induced by overloading pressure.

• Authors: Xiang-chun Shen, Zhi-yu Qian
• Journal: Yao xue xue bao = Acta pharmaceutica Sinica (Yao Xue Xue Bao) Vol. 39 Issue 3 Pg. 172-5 (Mar 2004) ISSN: 0513-4870 [Print] China
• PMID: 15171649 (Publication Type: Journal Article)

Chemical References

• trans-sodium crocetinate
• Vitamin A
• Carotenoids
• Matrix Metalloproteinase 2
• Matrix Metalloproteinase 9
• Ca(2+) Mg(2+)-ATPase
• Sodium-Potassium-Exchanging ATPase
• Hydroxyproline

Topics

• Animals
• Ca(2+) Mg(2+)-ATPase (metabolism)
• Cardiomegaly (enzymology, metabolism)
• Carotenoids (pharmacology)
• Hydroxyproline (metabolism)
• Male
• Matrix Metalloproteinase 2 (metabolism)
• Matrix Metalloproteinase 9 (metabolism)
• Myocardium (enzymology)
• Organ Size (drug effects)
• Rats
• Rats, Sprague-Dawley
• Sodium-Potassium-Exchanging ATPase (metabolism)
• Vitamin A (analogs & derivatives)