Abstract | AIM: METHODS:
Interleukin-1 production was measured by thymocyte co-stimulating assay; the radioactivity of [(3)H] arachidonic acid (AA) was used to evaluate the release of AA; prostaglandin E2 production was measured with radioimmunoassay (RIA); IL-2 and IFN-gamma were detected by ELISA method. RESULTS: EsA (3-12 micromol/L)could potently inhibit the production of IL-1 and PGE(2) from both silent and LPS induced macrophages. EsA had no significant effect on the release of AA from murine macrophages. EsA could inhibit the production of IL-2 from murine lymphocytes induced by ConA, but not affect the production from silent lymphocytes. EsA showed no effect on the production of IFN-gamma from both silent and ConA induced lymphocytes. CONCLUSION: EsA could affect the immunological function through inhibiting the production of IL-2 from activated splenocytes and the inhibition of production of IL-1 and PGE(2) might be one of the anti- inflammation mechanisms of EsA.
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Authors | Zhen-Yu Xiao, Qin-Yue Zheng, Yuan-Ying Jiang, Bin Zhou, Ming Yin, Hong-Bin Wang, Jun-Ping Zhang |
Journal | Acta pharmacologica Sinica
(Acta Pharmacol Sin)
Vol. 25
Issue 6
Pg. 817-21
(Jun 2004)
ISSN: 1671-4083 [Print] United States |
PMID | 15169638
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Anti-Inflammatory Agents, Non-Steroidal
- Drugs, Chinese Herbal
- Interleukin-1
- Interleukin-2
- Saponins
- Arachidonic Acid
- esculentoside A
- Oleanolic Acid
- Interferon-gamma
- Dinoprostone
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Topics |
- Animals
- Anti-Inflammatory Agents, Non-Steroidal
(pharmacology)
- Arachidonic Acid
(metabolism)
- Dinoprostone
(biosynthesis)
- Drugs, Chinese Herbal
(pharmacology)
- Female
- Interferon-gamma
(biosynthesis)
- Interleukin-1
(biosynthesis)
- Interleukin-2
(biosynthesis)
- Lymphocytes
(metabolism)
- Macrophages, Peritoneal
(metabolism)
- Male
- Mice
- Mice, Inbred BALB C
- Mice, Inbred ICR
- Oleanolic Acid
(analogs & derivatives, isolation & purification, pharmacology)
- Phytolacca
(chemistry)
- Plants, Medicinal
(chemistry)
- Saponins
(isolation & purification, pharmacology)
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