Abstract | PURPOSE: EXPERIMENTAL DESIGN: We evaluated 49 bladder cancer specimens for LRAT protein expression using immunohistochemistry with affinity-purified antibodies to human LRAT. LRAT mRNA expression was assessed using reverse transcription-PCR in bladder specimens from an additional 16 patients. We examined the effect of retinoic acid and ROL on LRAT mRNA expression in five human bladder cancer cell lines. RESULTS:
LRAT protein was detected throughout the nonneoplastic bladder epithelium in all of the specimens. In bladder tumors, LRAT protein expression was reduced compared with the nonneoplastic epithelium or was completely absent in 7 of 32 (21.9%) superficial tumors versus 16 of 17 (94.1%) invasive tumors (P < 0.001). All of the non-neoplastic bladder specimens tested (11 of 11) showed LRAT mRNA expression, compared with 5 of 8 (62%) superficial tumors and 0 of 5 (0%) invasive tumors (P = 0.001). Three of five human bladder cancer cell lines expressed LRAT mRNA independent of retinoid exposure, whereas in two cell lines LRAT mRNA expression was induced by retinoid treatment. CONCLUSIONS: We report a significant reduction in LRAT expression in bladder cancer. Moreover, we demonstrate an inverse correlation of LRAT mRNA and protein expression with increasing tumor stage. These data suggest that loss of LRAT expression is associated with invasive bladder cancer.
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Authors | Stephen Boorjian, Satish K Tickoo, Nigel P Mongan, Huayin Yu, Dean Bok, Robert R Rando, David M Nanus, Douglas S Scherr, Lorraine J Gudas |
Journal | Clinical cancer research : an official journal of the American Association for Cancer Research
(Clin Cancer Res)
Vol. 10
Issue 10
Pg. 3429-37
(May 15 2004)
ISSN: 1078-0432 [Print] United States |
PMID | 15161698
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Esters
- RNA, Messenger
- Receptors, Estrogen
- Receptors, Progesterone
- Tretinoin
- Acyltransferases
- lecithin-retinol acyltransferase
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Topics |
- Acyltransferases
(biosynthesis)
- Adult
- Aged
- Breast Neoplasms
(pathology)
- Carcinoma
(pathology)
- Cell Line, Tumor
- Disease Progression
- Esters
(metabolism)
- Extracellular Matrix
(metabolism)
- Female
- Humans
- Immunohistochemistry
- Male
- Middle Aged
- Multivariate Analysis
- Neoplasm Metastasis
- Oligonucleotide Array Sequence Analysis
- Prognosis
- Proportional Hazards Models
- RNA, Messenger
(metabolism)
- Receptors, Estrogen
(metabolism)
- Receptors, Progesterone
(metabolism)
- Reverse Transcriptase Polymerase Chain Reaction
- Risk
- Risk Factors
- Tretinoin
(pharmacology)
- Urinary Bladder Neoplasms
(enzymology)
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