[Study of antibody enzyme with acetylcholonesteraseactivity].

Abstract

AIM:

To study anti-idiotype antibody(AId Ab)directed at mAb 3F3 Fab idiotypic determinant with acetylcholinesteras(AchE) activity.

METHODS:

Anti-AchE mAb 3F3(IgG1) was digested by papain to prepare its Fab fragments. And then the Fab fragments were purified through Ach- and SPA-Sepharose 4B columns respectively. The BALB/c mice were immunized by using purified Fab fragments as immunogen to prepare AId Abs against the Fab fragmrnts. Catalytic activity of the AId Abs was detected by ELISA.

RESULTS:

The purified mAb 3F3 Fab fragments were acquired by papain digestion and affinity chromatograpy successively. Enzyme catalysis ELISA detection showed that AId Abs to the Fab fragments had activity of AchE.

CONCLUSION:

An AId Ab to mAb 3F3 Fab fragments has been prepared successfully, which opens up a novel way for treatment of pesticides poisoning.

• Authors: Mei-Hua Gao, Feng-Hua Fu, Ying Cheng, Zhao-Long Gong, Tao Hu
• Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi) Vol. 19 Issue 3 Pg. 255-6 (May 2003) ISSN: 1007-8738 [Print] China
• PMID: 15155086 (Publication Type: Journal Article)

Chemical References

• Antibodies, Monoclonal
• Antigens
• Immunoglobulin Fab Fragments
• Immunoglobulin G

Topics

• Animals
• Antibodies, Monoclonal (immunology)
• Antigens
• Immunoglobulin Fab Fragments (immunology)
• Immunoglobulin G (immunology)
• Mice, Inbred BALB C