Abstract | AIMS: METHODS AND RESULTS: The spaA gene of Erysipelothrix rhusiopathiae was inserted into a shuttle plasmid pSECE1 to construct pSECE1.3. The SpaA produced in L. lactis maintained a stable antigenicity without degrading in growth. After mice were inoculated intranasally and orally with pSECE1.3-carrying L. lactis cells, IgG and IgA specific to SpaA were detected, and all the mice survived a challenge with 100 LD(50) of E. rhusiopathiae Tama-96 in the inner thigh. CONCLUSIONS: SIGNIFICANCE AND IMPACT OF THE STUDY: In this vaccination system, purification of the antigen and injection are unnecessary, leading to a reduced production cost, reduced labour and less stress to the animals. This vaccination system of the lactic acid bacteria should be a safe and suitable vehicle for a polyvalent vaccine.
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Authors | H I Cheun, K Kawamoto, M Hiramatsu, H Tamaoki, T Shirahata, S Igimi, S-I Makino |
Journal | Journal of applied microbiology
(J Appl Microbiol)
Vol. 96
Issue 6
Pg. 1347-53
( 2004)
ISSN: 1364-5072 [Print] England |
PMID | 15139928
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Bacterial
- Antigens, Bacterial
- Bacterial Proteins
- Bacterial Vaccines
- Immunoglobulin A
- Immunoglobulin G
- SpaA protein, Erysipelothrix rhusiopathiae
- Vaccines, DNA
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Topics |
- Administration, Intranasal
- Animals
- Antibodies, Bacterial
(blood)
- Antigens, Bacterial
(immunology)
- Bacterial Proteins
(immunology)
- Bacterial Vaccines
(immunology)
- Female
- Immunoglobulin A
(blood)
- Immunoglobulin G
(blood)
- Lactococcus lactis
(immunology)
- Mice
- Plasmids
- Swine
- Swine Erysipelas
(immunology, prevention & control)
- Vaccination
(methods)
- Vaccines, DNA
(immunology)
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