Differentially expressed genes in multidrug resistant variants of U-2 OS human osteosarcoma cells.

Abstract	Multidrug resistance (MDR) to anticancer agents is a major barrier to the successful treatment of human osteosarcomas. Current understanding of the genes that contribute to the features of MDR is limited, and the mechanisms remain unclear. Here we applied differential display reverse transcription-polymerase chain reaction (DDRT-PCR) to parental and MDR-variants of U-2 OS human osteosarcoma cells, to clarify the genes involved in the MDR cells, and identified five candidate genes. These are BCRP (breast cancer resistance protein) encoding a transmembrane efflux pump; RB1CC1 (RB1-inducible coiled-coil 1), a tumor suppressor regulating RB1 (retinoblastoma 1) expression; a novel transcriptional variant of dUTPase; SSR2 (beta-signal sequence receptor), which is associated with protein translocation across ER membrane; and HSP105 encoding high molecular mass heat shock proteins. Molecular and biological characterization of these genes will yield further insight into the features between MDR and tumor progression in human osteosarcomas.
Authors	Tokuhiro Chano, Kanji Mori, Katia Scotlandi, Stefania Benini, Cristina Lapucci, Maria Cristina Manara, Massimo Serra, Piero Picci, Hidetoshi Okabe, Nicola Baldini
Journal	Oncology reports (Oncol Rep) Vol. 11 Issue 6 Pg. 1257-63 (Jun 2004) ISSN: 1021-335X [Print] Greece
PMID	15138564 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	ATP Binding Cassette Transporter, Subfamily B, Member 1 Neoplasm Proteins Doxorubicin
Topics	ATP Binding Cassette Transporter, Subfamily B, Member 1 (genetics, metabolism) Amino Acid Sequence Base Sequence Bone Neoplasms (genetics, metabolism, pathology) Doxorubicin (pharmacology) Drug Resistance, Multiple (genetics) Drug Resistance, Neoplasm (genetics) Gene Expression Profiling Gene Expression Regulation, Neoplastic Humans Molecular Sequence Data Neoplasm Proteins (genetics) Osteosarcoma (genetics, metabolism, pathology) Polymerase Chain Reaction Subtraction Technique Tumor Cells, Cultured (drug effects, metabolism)

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