Abstract | BACKGROUND/PURPOSE: METHODS: Adult rats underwent intestinal ischemia-reperfusion (IIR), 60-minute ischemia and 60-minute reperfusion, or sham (120 minutes) at either normothermia or moderate hypothermia. Four groups of animals were studied: (1) normothermic sham (NS), (2) normothermic IIR (NIIR), (3) hypothermic sham (HS), and (4) hypothermic IIR (HIIR). Western blotting measured heat shock protein expression, phosphorylated (p-) and total (T-) hepatic STAT-1 and STAT-3. RESULTS: There were no differences in expression of HSPs 27, 47, 60, i70, c70, or 90 between any of the experimental groups. NIIR caused a significant increase in p-STAT-1 compared with normothermic sham (P <.05) and a highly significant increase in p-STAT-3 (P <.001), both these increases were completely abolished by moderate hypothermia (P <.01 v NIIR.) CONCLUSIONS: The protective effect of moderate hypothermia on liver is not mediated by HSP expression at this time-point. Hypothermia may act by decreasing hepatic STAT activation, supporting the potential therapeutic role of moderate hypothermia. Modulation of STAT activation may also provide novel therapeutic targets.
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Authors | E J Parkinson, P A Townsend, A Stephanou, D S Latchman, S Eaton, A Pierro |
Journal | Journal of pediatric surgery
(J Pediatr Surg)
Vol. 39
Issue 5
Pg. 696-701
(May 2004)
ISSN: 1531-5037 [Electronic] United States |
PMID | 15137002
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- DNA-Binding Proteins
- Heat-Shock Proteins
- STAT1 Transcription Factor
- STAT3 Transcription Factor
- Stat1 protein, rat
- Stat3 protein, rat
- Trans-Activators
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Topics |
- Animals
- DNA-Binding Proteins
(metabolism)
- Disease Models, Animal
- Heat-Shock Proteins
(metabolism)
- Hypothermia, Induced
- Liver
(metabolism)
- Male
- Phosphorylation
- Rats
- Rats, Sprague-Dawley
- Reperfusion Injury
(metabolism, therapy)
- STAT1 Transcription Factor
- STAT3 Transcription Factor
- Signal Transduction
- Trans-Activators
(metabolism)
- Transcriptional Activation
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