Abstract |
We recently found that a small molecule 2[[3-(2,3-dichlorophenoxy)propyl]amino] ethanol (2,3-DCPE) could induce apoptosis and downregulate Bcl-XL expression in various cancer cells. Here, we found that 2,3-DCPE suppressed the proliferation of Bcl-XL-overexpressing cancer cells without inducing apoptosis. Subsequently, we found that 2,3-DCPE could induce S-phase arrest and upregulate p21 but not p27 at a time- and dose-dependent but p53-dispensable manner in DLD-1 human colon cancer cells. Activation of ERK was also detected after treatment with 2,3-DCPE. Moreover, p21 induction was dramatically attenuated by ERK inhibitors PD98059 and U0126. Induction of p21 and S-phase arrest and corresponding activation of ERK were also observed in ATM-defective cells, suggesting that 2,3-DCPE-induced these events were ATM-dispensable. Furthermore, ERK inhibitors dramatically attenuated 2,3-DCPE-induced S-phase arrest. Together, our data indicate that ERK activation correlated with the 2,3-DCPE-mediated induction of p21 expression and S-phase arrest. This finding may have implication for cancer therapy.
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Authors | Hongbo Zhu, Lidong Zhang, Shuhong Wu, Fuminori Teraishi, John J Davis, Dietmar Jacob, Bingliang Fang |
Journal | Oncogene
(Oncogene)
Vol. 23
Issue 29
Pg. 4984-92
(Jun 24 2004)
ISSN: 0950-9232 [Print] England |
PMID | 15122344
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- 2-((3-(2,3-dichlorophenoxy)propyl)amino)ethanol
- CDKN1A protein, human
- Cell Cycle Proteins
- Chlorobenzenes
- Cyclin-Dependent Kinase Inhibitor p21
- Cyclins
- DNA-Binding Proteins
- Ethanolamines
- Proto-Oncogene Proteins c-bcl-2
- Tumor Suppressor Protein p53
- Tumor Suppressor Proteins
- ATM protein, human
- Ataxia Telangiectasia Mutated Proteins
- Protein Serine-Threonine Kinases
- Mitogen-Activated Protein Kinases
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Topics |
- Apoptosis
- Ataxia Telangiectasia Mutated Proteins
- Cell Cycle Proteins
- Cell Line, Tumor
- Chlorobenzenes
(pharmacology)
- Cyclin-Dependent Kinase Inhibitor p21
- Cyclins
(metabolism)
- DNA-Binding Proteins
- Down-Regulation
- Ethanolamines
(pharmacology)
- Gene Expression Regulation, Neoplastic
- Humans
- Mitogen-Activated Protein Kinases
(metabolism)
- Protein Serine-Threonine Kinases
(metabolism)
- Proto-Oncogene Proteins c-bcl-2
(metabolism)
- S Phase
- Tumor Suppressor Protein p53
(metabolism)
- Tumor Suppressor Proteins
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