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Nano red elemental selenium has no size effect in the induction of seleno-enzymes in both cultured cells and mice.

Abstract
We previous reported that a nano red elemental selenium (Nano-Se) in the range from 20 approximately 60 nm had similar bioavailability to sodium selenite (BioFactors 15 (2001) 27). We recently found that Nano-Se with different size had marked difference in scavenging an array of free radicals in vitro, the smaller the particle, the better scavenging activity (Free Radic. Biol. Med. 35 (2003) 805). In order to examine whether there is a size effect of Nano-Se in the induction of Se-dependent enzymes, a range of Nano-Se (5 approximately 200 nm) have been prepared based on the control of elemental Se atom aggregation. The sizes of Nano-Se particles were inversely correlated with protein levels in the redox system of selenite and glutathione. Different sizes of red elemental Se were prepared by adding varying amount of bovine serum albumin (BSA). Three different sizes of Nano-Se (5 approximately 15 nm, 20 approximately 60 nm, and 80 approximately 200 nm) have been chosen for the comparison of biological activity in terms of the induction of seleno-enzyme activities. Results showed that there was no significant size effect of Nano-Se from 5 to 200 nm in the induction of glutathione peroxidase (GPx), phospholipid hydroperoxide glutathione peroxidase (PHGPx) and thioredoxin reductase-1 (TrxR-1) in human hepatoma HepG2 cells and the livers of mice.
AuthorsJinsong Zhang, Huali Wang, Yongping Bao, Lide Zhang
JournalLife sciences (Life Sci) Vol. 75 Issue 2 Pg. 237-44 (May 28 2004) ISSN: 0024-3205 [Print] Netherlands
PMID15120575 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Fluorescent Dyes
  • Serum Albumin, Bovine
  • Phospholipid Hydroperoxide Glutathione Peroxidase
  • Glutathione Peroxidase
  • TXNRD1 protein, human
  • Thioredoxin Reductase 1
  • Thioredoxin-Disulfide Reductase
  • Txnrd1 protein, mouse
  • Selenium
Topics
  • Animals
  • Enzyme Induction (drug effects)
  • Fluorescent Dyes (metabolism)
  • Glutathione Peroxidase (biosynthesis)
  • Humans
  • Liver (enzymology)
  • Mice
  • Microscopy, Electron
  • Particle Size
  • Phospholipid Hydroperoxide Glutathione Peroxidase
  • Selenium (metabolism, pharmacology)
  • Serum Albumin, Bovine (metabolism)
  • Thioredoxin Reductase 1
  • Thioredoxin-Disulfide Reductase (biosynthesis)
  • Tumor Cells, Cultured

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