Could prolactin receptor gene polymorphism play a role in pathogenesis of breast carcinoma?

Abstract

OBJECTIVE:

Constitutive activation of various hormone and growth factor receptors is newly recognised as a common cause of tumour development. This study investigated the presence of any mutation or polymorphism of prolactin receptor (PRLR) in 38 patients with breast cancer.

RESEARCH METHODS:

Genomic DNA was extracted and PCR amplification was carried out for exon 1-10 of PRLR from tumoral and adjacent non-cancerous breast tissue of tumour specimens from 38 breast cancer patients. PCR products were analysed by SSCP and automatic sequencing for mutations.

RESULTS:

For the first time, A150C (Leu-->Ile) transversion at exon 6 of PRLR in tumour tissues, in adjacent non-cancerous breast tissues, and in blood samples of two (5.3%) out of 38 patients with breast cancer were detected. In contrast to this finding, no polymorphism of PRLR in blood samples of 100 normal individuals were found.

CONCLUSION:

Polymorphism of prolactin receptors might play a role in mammary carcinogenesis as a consequence of intracellular changes of PRLR signalling.

• Authors: Emel Canbay, Naci Degerli, Bahadir M Gulluoglu, Handan Kaya, Metin Sen, Fevzi Bardakci
• Journal: Current medical research and opinion (Curr Med Res Opin) Vol. 20 Issue 4 Pg. 533-40 (Apr 2004) ISSN: 0300-7995 [Print] England
• PMID: 15119991 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Receptors, Estrogen
• Receptors, Progesterone
• Receptors, Prolactin

Topics

• Adult
• Breast Neoplasms (genetics, metabolism)
• Exons
• Female
• Humans
• Middle Aged
• Mutation
• Polymerase Chain Reaction
• Polymorphism, Genetic
• Polymorphism, Single-Stranded Conformational
• Receptors, Estrogen (analysis)
• Receptors, Progesterone (analysis)
• Receptors, Prolactin (genetics)
• Sequence Analysis, DNA