Complement component C3a or C3a desArg as a new marker for estimation of local vital reactions in incised skin wounds.

Abstract

The anaphylatoxin C3a or its desArg form (C3a/desArg) generated during complement activation could be detected in the vicinity of incised skin wounds of guinea pigs using immunoblotting methods. The C3a/desArg peptides were detectable immediately after injury in local sites up to 3 mm from the wound edge. In subsequent determinations of up to at least 3-day-old antemortem wounds, the maximum concentration of these peptides was largely localized up to 6 mm from the wound edge at 2 h after injury. In postmortem wounds, however, these peptides were undetectable. When they were released in antemortem wounded tissues they could be detected up to 1 day at 22 degrees C after death. These results suggest that the detection of C3a/desArg in wounds using immunoblotting methods can be useful for distinguishing ante- from postmortem wounds.

• Authors: Y Maeno, Y Mori, M Iwasa, H Inoue, F Takabe
• Journal: Forensic science international (Forensic Sci Int) Vol. 55 Issue 1 Pg. 37-44 (Jul 1992) ISSN: 0379-0738 [Print] Ireland
• PMID: 1511937 (Publication Type: Journal Article)

Chemical References

• Biomarkers
• complement C3a, des-Arg-(77)-
• Complement C3a

Topics

• Animals
• Biomarkers
• Complement C3a (analysis)
• Electrophoresis, Polyacrylamide Gel
• Guinea Pigs
• Immunoblotting
• Male
• Molecular Weight
• Postmortem Changes
• Skin (chemistry)