Sensitive enzyme-linked immunosorbent assay with urine samples: a tool for surveillance of schistosomiasis japonica.

Abstract

An enzyme-linked immunosorbent assay (ELISA) to detect antibodies to Schistosoma japonicum soluble egg antigens (SEA) in un-concentrated urine was developed. The urine ELISA was applied to samples collected in a schistosomiasis-endemic village in China. The levels of anti-SEA antibodies detected in urine correlated well with those obtained with paired serum samples (r = 0.694, p<0.0001). Among 129 serum ELISA positives, 112 (86.8%) were positive by urine ELISA, while all 40 serum ELISA negatives from a non-endemic area were negative. The levels of anti-SEA in urine samples were stable up to 8 weeks of storage at 37 degrees C, with sodium azide as a preservative. Therefore, ELISA with urine samples can be used for the surveillance of schistosomiasis.

• Authors: Makoto Itoh, Nobuo Ohta, Tamotsu Kanazawa, Yasuo Nakajima, Masanori Sho, Masaru Minai, Zhou Daren, Yan Chen, Hongbin He, Yong-Kang He, Zhinan Zhong
• Journal: The Southeast Asian journal of tropical medicine and public health (Southeast Asian J Trop Med Public Health) Vol. 34 Issue 3 Pg. 469-72 (Sep 2003) ISSN: 0125-1562 [Print] Thailand
• PMID: 15115114 (Publication Type: Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Helminth
• Immunoglobulin G

Topics

• Antibodies, Helminth (urine)
• Enzyme-Linked Immunosorbent Assay (methods)
• Humans
• Immunoglobulin G (urine)
• Population Surveillance (methods)
• Schistosomiasis japonica (diagnosis, prevention & control)
• Sensitivity and Specificity
• Specimen Handling