Abstract | BACKGROUND: METHODS: We determined activities of XOR forms, polymorphonuclear elastase (PMN-E), aminotransferases, and hyaluronic acid in plasma of 20 patients undergoing LT. Samples were taken from the radial artery (RA) before the anhepatic phase; from the portal vein (PV) before reperfusion; from graft caval effluent (CE) at reperfusion; and from RA, PV, and the hepatic vein (HV) 10 and 90 min postreperfusion. RESULTS: The graft, but not recipient bowel, released XOR into blood (XOR in CE, median, 61.2 mU/ g protein [range, 1.9-160.4 vs. undetectable in PV before reperfusion). Circulating XOR was transformed from dehydrogenase to reversible oxidase (XOrev) (XOrev-to-XOR ratio, 48.1% in CE and 65.1% in HV 90 min postreperfusion). Neutrophil activation was detected in the recipients before reperfusion, and in liver at early post-reperfusion (median PMN-E was 0.85 microg/ g protein [range, 0.01-1.58] in RA before the anhepatic phase; 2.22 microg/ g protein [range, 0.20-5.88] in PV prereperfu-sion; and 3.60 microg/ g protein [range, 0.48-6.78] in HV 10 min postreperfusion). XOR, but none of the other markers, was higher in the CE of patients with moderate primary graft dysfunction than in those with slight primary graft dysfunction. CONCLUSIONS: XOR release and neutrophil activation are produced during LT, and they are potentially injurious mechanisms associated with this therapy.
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Authors | Ramon Martí, Enric Múrio, Encarna Varela, Itxarone Bilbao, Carles Pascual, Carlos Margarit, Rosa M Segura |
Journal | Transplantation
(Transplantation)
Vol. 77
Issue 8
Pg. 1239-45
(Apr 27 2004)
ISSN: 0041-1337 [Print] United States |
PMID | 15114092
(Publication Type: Journal Article)
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Chemical References |
- Xanthine Oxidase
- Leukocyte Elastase
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Topics |
- Adult
- Aged
- Humans
- Leukocyte Elastase
(metabolism)
- Liver
(enzymology, injuries)
- Liver Transplantation
(adverse effects, immunology, physiology)
- Middle Aged
- Neutrophil Activation
- Organ Preservation
(adverse effects)
- Xanthine Oxidase
(metabolism)
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