AT-rich promoter elements of soybean heat shock gene Gmhsp17.5E bind two distinct sets of nuclear proteins in vitro.

Abstract

A 33 bp double-stranded oligonucleotide homologous to two AT-rich sequences located upstream (-907 to -889 and -843 to -826) to the start of transcription of heat shock gene Gmhsp17.5E of soybean stimulated transcription when placed 5' to a truncated (-140) maize Adh1 promoter. The chimeric promoter was assayed in vivo utilizing anaerobically stressed sunflower tumors transformed by a pTi-based vector of Agrobacterium tumefaciens. Nuclear proteins extracted from soybean plumules were shown to bind double-stranded oligonucleotides homologous to AT-rich sequences in the 5' flanking regions of soybean beta-conglycinin, lectin, leghemoglobin and heat shock genes. These proteins were also shown to bind AT-rich probes homologous to homeobox protein binding sites from the Antennapedia and engrailed/fushi tarazu genes of Drosophila. Binding activity specific for AT-rich sequences showed a wide distribution among various plant organs and species. Preliminary characterization indicated that two sets of nuclear proteins from soybean bind AT-rich DNA sequences: a diverse high-molecular-weight (ca. 46-69 kDa) group, and a low-molecular-weight (23 and 32 kDa) group of proteins. The latter meets the operational criteria for high-mobility group proteins (HMGs).

• Authors: E Czarnecka, J C Ingersoll, W B Gurley
• Journal: Plant molecular biology (Plant Mol Biol) Vol. 19 Issue 6 Pg. 985-1000 (Sep 1992) ISSN: 0167-4412 [Print] Netherlands
• PMID: 1511143 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Heat-Shock Proteins
• High Mobility Group Proteins
• Nuclear Proteins
• Alcohol Dehydrogenase

Topics

• Alcohol Dehydrogenase (genetics)
• Base Composition
• Base Sequence
• Cloning, Molecular
• Heat-Shock Proteins (genetics)
• High Mobility Group Proteins (metabolism)
• Molecular Sequence Data
• Nuclear Proteins (isolation & purification, metabolism)
• Promoter Regions, Genetic (genetics)
• Glycine max (genetics)
• Zea mays (enzymology, genetics)