Abstract |
We examined metabolism of PbTxs in New Zealand cockle, Austrovenus (A.) stutchburyi, and greenshell mussel, Perna (P.) canaliculus, by means of liquid chromatography coupled with tandem mass spectrometry. PbTx-2, PbTx-3 and BTX-B5 were detected in Karenia (K.) brevis culture medium in the ratio of ca. 50:2:5. The amounts of PbTx-3 and BTX-B5 were greatly increased in both seawater and shellfish exposed to K. brevis cultures or supernatant prepared by disruption of K. brevis under appropriate condition, while those of PbTx-2 were decreased. Some PbTx-2 was present in P. canaliculus, but not in A. stutchburyi. Low levels of BTX-B1 were detected in A. stutchburyi, but not P. canaliculus. Levels of PbTx-3 and BTX-B5 were highest immediately after exposure and then declined rapidly in both shellfish. BTX-B1 increased in concentration after exposure, and was then gradually eliminated from A. stutchburyi. Three successive exposures of A. stutchburyi to K. brevis cultures resulted in similar initial levels of PbTx-3 and BTX-B5, while BTX-B1 accumulated after each dose. In P. canaliculus, initial levels of PbTx-3 were similar, while PbTx-2 and BTX-B5 accumulated after each dose. PbTx-3 and BTX-B5 are proposed to be suitable markers for monitoring shellfish toxicity after a red tide event.
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Authors | Hitoshi Ishida, Akira Nozawa, Haruo Nukaya, Lesley Rhodes, Paul McNabb, Patrick T Holland, Kuniro Tsuji |
Journal | Toxicon : official journal of the International Society on Toxinology
(Toxicon)
Vol. 43
Issue 6
Pg. 701-12
(May 2004)
ISSN: 0041-0101 [Print] England |
PMID | 15109891
(Publication Type: Journal Article)
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Chemical References |
- Marine Toxins
- Neurotoxins
- Oxocins
- brevetoxin
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Topics |
- Animals
- Bivalvia
(drug effects, metabolism)
- Dinoflagellida
- Foodborne Diseases
(etiology)
- Marine Toxins
(pharmacokinetics, toxicity)
- Neurotoxins
(pharmacokinetics, toxicity)
- Oxocins
(pharmacokinetics, toxicity)
- Shellfish
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