ADP-ribosylation by exoenzyme T of Pseudomonas aeruginosa induces an irreversible effect on the host cell cytoskeleton in vivo.

Abstract	Pseudomonas aeruginosa utilises a type III secretion system (TTSS) to introduce exoenzyme S and exoenzyme T into host cells to subvert host cell signalling and thereby promote infection. In this study, we have employed the heterologous TTSS of Yersinia to deliver different mutants of ExoT into HeLa cells. Wild-type ExoT and ExoT variants expressing either GAP (GTPase activating protein) or ADP-ribosyltransferase activity mediated changes in cell morphology, which correlated to disruption of the actin microfilaments of the infected cells. ExoT expressing ADP-ribosylating activity gave an irreversible effect on HeLa cell morphology, while ExoT expressing only GAP activity displayed a reversible effect where the cells regained normal cell morphology after killing of the infecting bacteria. This shows that ExoT can modify and inactivate host cell proteins involved in maintaining the actin cytoskeleton in vivo by two independent mechanisms.
Authors	Charlotta Sundin, Bengt Hallberg, Ake Forsberg
Journal	FEMS microbiology letters (FEMS Microbiol Lett) Vol. 234 Issue 1 Pg. 87-91 (May 01 2004) ISSN: 0378-1097 [Print] England
PMID	15109724 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Actins Bacterial Proteins ExoT protein, Pseudomonas aeruginosa GTPase-Activating Proteins Recombinant Proteins Adenosine Diphosphate Ribose ADP Ribose Transferases
Topics	ADP Ribose Transferases (genetics, metabolism) Actin Cytoskeleton (metabolism, ultrastructure) Actins (metabolism) Adenosine Diphosphate Ribose (metabolism) Bacterial Proteins (genetics, metabolism) Cell Line Cell Size Cloning, Molecular Cytoskeleton (metabolism, ultrastructure) GTPase-Activating Proteins Gene Expression Genes, Bacterial HeLa Cells Humans Microscopy Microscopy, Fluorescence Mutation Protein Structure, Tertiary Protein Transport Pseudomonas aeruginosa (enzymology, pathogenicity) Recombinant Proteins (metabolism) Signal Transduction Yersinia pseudotuberculosis (genetics, metabolism)

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