Abstract | PURPOSE: To study the molecular characteristics of lens epithelial cells from patients with senile cataract by cDNA microarray technique. METHODS: Lens epithelial cells adhering to anterior capsules taken during cataract surgery collected from 108 patients, aged 56-92 years (senile cataract group), were pooled. Pooled epithelial cells of normal, noncataractous lenses from one patient with ocular trauma, one patient with lens subluxation, and 25 cadaveric eyes, all under the age of 55 years, served as a control. Total RNA was extracted by conventional methods from the two groups of cells, and a fluorescent probe was prepared for each group. The probes were hybridized on 9700 known human cDNA clones. Hybridized clones were analysed using a scanning laser and the results were processed by GEMTools (Incyte Genomics) software. RESULTS: CONCLUSIONS: Cataractogenesis is associated with numerous changes in the genetic profile of the lens epithelial cells. Since filensin, IRPC, and PEDF genes are known to have important roles in the physiology and morphology of the transparent lens, substantial downregulation of their expression might contribute to the formation of senile cataract.
|
Authors | F Segev, O Mor, A Segev, M Belkin, E I Assia |
Journal | Eye (London, England)
(Eye (Lond))
Vol. 19
Issue 1
Pg. 80-5
(Jan 2005)
ISSN: 0950-222X [Print] England |
PMID | 15105821
(Publication Type: Journal Article)
|
Chemical References |
- DNA Probes
- DNA, Complementary
- Eye Proteins
- Intermediate Filament Proteins
- Nerve Growth Factors
- Potassium Channels, Inwardly Rectifying
- RNA, Messenger
- Serpins
- filensin
- pigment epithelium-derived factor
- RNA
|
Topics |
- Aged
- Aged, 80 and over
- Aging
(genetics)
- Cataract
(genetics)
- DNA Probes
(genetics)
- DNA, Complementary
(genetics)
- Down-Regulation
(genetics)
- Epithelial Cells
- Eye Proteins
(genetics)
- Gene Expression Regulation
(genetics)
- Humans
- Intermediate Filament Proteins
(genetics)
- Lens, Crystalline
(pathology)
- Middle Aged
- Nerve Growth Factors
(genetics)
- Oligonucleotide Array Sequence Analysis
(methods)
- Potassium Channels, Inwardly Rectifying
(genetics)
- RNA
(analysis)
- RNA, Messenger
(analysis)
- Serpins
(genetics)
|