Metabolism of eupatilin in rats using liquid chromatography/electrospray mass spectrometry.

Abstract

Eupatilin (5,7-dihydroxy-3',4',6-trimethoxy flavone) is an active ingredient of an ethanol extract of Artemisia asiatica (DA-9601) that is used in the treatment of gastritis. In vitro and in vivo metabolism of eupatilin in the rats has been studied by LC-electrospray mass spectrometry. Rat liver microsomal incubation of eupatilin in the presence of NADPH and UDPGA resulted in the formation of four metabolites (M1-M4). M1, M2, M3 and M4 were tentatively identified as 3'- or 4'-O-demethyl-eupatilin glucuronide, eupatilin glucuronide, 6-O-demethyleupatilin and 3'- or 4'-O-demethyl-eupatilin, respectively. Those metabolites from in vitro study were also characterized in bile, plasma or urine samples after an intravenous administration of eupatilin to rats. In rat bile, plasma and urine samples, eupatilin glucuronide (M2) was a major metabolite, whereas M3, M4 and M4 glucuronide (M1) were the minor metabolites.

• Authors: Hye Young Ji, Hye Won Lee, Hyun Joo Shim, Soon Hoe Kim, Won Bae Kim, Hye Suk Lee
• Journal: Biomedical chromatography : BMC (Biomed Chromatogr) Vol. 18 Issue 3 Pg. 173-7 (Apr 2004) ISSN: 0269-3879 [Print] England
• PMID: 15103703 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright 2004 John Wiley & Sons, Ltd.

Chemical References

• Drugs, Chinese Herbal
• Flavonoids
• eupatilin

Topics

• Animals
• Chromatography, Liquid (methods)
• Drugs, Chinese Herbal
• Flavonoids (metabolism)
• Male
• Microsomes, Liver (metabolism)
• Rats
• Spectrometry, Mass, Electrospray Ionization (methods)