Abstract |
We aimed to investigate the effects of electric shock (ES) on the course of experimental colitis and the involvement of possible central and peripheral mechanisms. In Sprague-Dawley rats (n = 190) colitis was induced by intracolonic administration 2,4,6-trinitrobenzenesulfonic acid (TNBS). The effects of ES (0.3-0.5 mA) or the central administration of corticotropin-releasing factor (CRF; astressin, 10 microg/kg) or cholecystokinin (CCKB; 20 microg/kg) receptor antagonists and peripheral glucocorticoid receptor ( RU-486; 10 mg/kg) or ganglion ( hexamethonium; 15 mg/kg) blockers on TNBS-induced colitis were studied by the assessment of macroscopic score, histological analysis and tissue myeloperoxidase activity. ES reduced all colonic damage scores (p < 0.05-0.01), while central CRF (p < 0.05-0.001) and CCKB receptor (p < 0.05-0.01) blockers or peripheral hexamethonium (p < 0.05-0.01) and RU-486 (p < 0.05) reversed stress-induced improvement. ES demonstrated an anti-inflammatory effect on colitis, which appears to be mediated by central CRF and CCK receptors with the participation of hypothalamo-pituitary-adrenal axis and the sympathetic nervous system.
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Authors | Mehmet Ali Gülpinar, Dilek Ozbeyli, Serap Arbak, Berrak C Yeğen |
Journal | Life sciences
(Life Sci)
Vol. 75
Issue 1
Pg. 77-91
(May 21 2004)
ISSN: 0024-3205 [Print] Netherlands |
PMID | 15102523
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Ganglionic Blockers
- Neurotransmitter Agents
- Receptor, Cholecystokinin B
- Mifepristone
- Hexamethonium
- Trinitrobenzenesulfonic Acid
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Topics |
- Acute Disease
- Animals
- Colitis
(chemically induced, metabolism, pathology)
- Colon
(drug effects, pathology)
- Disease Models, Animal
- Electric Stimulation
- Ganglionic Blockers
(administration & dosage, pharmacology)
- Hexamethonium
(administration & dosage, pharmacology)
- Hypothalamo-Hypophyseal System
(metabolism, physiology)
- Injections, Intraventricular
- Intestinal Mucosa
(drug effects, pathology)
- Male
- Mifepristone
(administration & dosage, pharmacology)
- Neurotransmitter Agents
(administration & dosage, pharmacology)
- Pituitary-Adrenal System
(metabolism, physiology)
- Rats
- Rats, Sprague-Dawley
- Receptor, Cholecystokinin B
(antagonists & inhibitors, metabolism)
- Restraint, Physical
- Stress, Physiological
(metabolism, physiopathology)
- Sympathetic Nervous System
(metabolism, physiology)
- Trinitrobenzenesulfonic Acid
(toxicity)
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