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Type I MOZ/CBP (MYST3/CREBBP) is the most common chimeric transcript in acute myeloid leukemia with t(8;16)(p11;p13) translocation.

Abstract
The t(8;16)(p11;p13) fuses the MOZ (MYST3) gene at 8p11 with CBP (CREBBP) at 16p13 and is associated with an infrequent but well-defined type of acute myeloid leukemia (AML) that has unique morphocytochemical findings (monocytoid blast morphology with erythrophagocytosis and simultaneously positive for myeloperoxidase and nonspecific esterases). RT-PCR amplification of MOZ/CBP (MYST3/CREBBP) chimera has proved difficult, with four different transcripts found in four reported cases. We studied 7 AML-t(8;16) patients, 5 with cytogenetically demonstrated t(8;16) and 2 with similar morphocytochemical and immunophenotypical characteristics. Clinically, 3 cases presented as therapy-related leukemia. Extramedullar involvement was observed at presentation in 2 patients and coagulopathy in 4. The clinicobiological findings confirmed the distinctiveness of this entity. Of note is the erythrophagocytosis in 5 of 7 cases and the immunological negativity for CD34 and CD117 and positivity for CD56. Using a new RT-PCR strategy, we were able to amplify a specific band of 212 bp in six cases in which sequence analysis confirmed the presence of the previously described MOZ/CBP fusion transcript type I. This is the largest molecularly studied AML-t(8;16) series, which demonstrates that MOZ/CBP breakpoints are usually clustered in intron 16 of MOZ and intron 2 of CBP. The newly designed single-round PCR provides a simple tool for the molecular confirmation of MOZ/CBP rearrangement.
AuthorsMaría Rozman, Mireia Camós, Dolors Colomer, Neus Villamor, Jordi Esteve, Dolors Costa, Ana Carrió, Marta Aymerich, Josep Lluis Aguilar, Alícia Domingo, Francesc Solé, Federico Gomis, Lourdes Florensa, Emili Montserrat, Elias Campo
JournalGenes, chromosomes & cancer (Genes Chromosomes Cancer) Vol. 40 Issue 2 Pg. 140-5 (Jun 2004) ISSN: 1045-2257 [Print] United States
PMID15101047 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright 2004 Wiley-Liss, Inc.
Chemical References
  • MOZ-CBP fusion protein, human
  • Neoplasm Proteins
  • Oncogene Proteins, Fusion
Topics
  • Acute Disease
  • Adult
  • Aged
  • Chromosome Breakage (genetics)
  • Chromosome Mapping
  • Chromosomes, Human, Pair 16 (genetics)
  • Chromosomes, Human, Pair 8 (genetics)
  • Female
  • Humans
  • Karyotyping
  • Leukemia, Myeloid (genetics)
  • Male
  • Middle Aged
  • Neoplasm Proteins (genetics)
  • Oncogene Proteins, Fusion (genetics)
  • Translocation, Genetic (genetics)

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