Identification of putative voltage-dependent Ca2+-permeable channels involved in cryptogein-induced Ca2+ transients and defense responses in tobacco BY-2 cells.

Ca(2+) is the pivotal second messenger for induction of defense responses induced by treatment of pathogen-derived elicitor or microbial infection in plants. However, molecular bases for elicitor-induced generation of Ca(2+) signals (Ca(2+) transients) are largely unknown. We here identified cDNAs for putative voltage-dependent Ca(2+)-permeable channels, NtTPC1A and NtTPC1B, that are homologous to TPC1 (two pore channel) from suspension-cultured tobacco BY-2 cells. NtTPC1s complemented the growth of a Saccharomyces cerevisiae mutant defective in CCH1, a putative Ca(2+) channel, in a low Ca(2+) medium, suggesting that both products permeate Ca(2+) through the plasma membrane. Cosuppression of NtTPC1s in apoaequorin-expressing BY-2 cells resulted in inhibition of rise in cytosolic free Ca(2+) concentration ([Ca(2+)](cyt)) in response to sucrose and a fungal elicitor cryptogein, while it did not affect hypoosmotic shock-induced [Ca(2+)](cyt) increase. Cosuppression of NtTPC1s also caused suppression of cryptogein-induced programmed cell death and defense-related gene expression. These results suggest that NtTPC1s are involved in Ca(2+) mobilization induced by the cryptogein and sucrose, and have crucial roles in cryptogein-induced signal transduction pathway.
AuthorsYasuhiro Kadota, Takuya Furuichi, Yoko Ogasawara, Tatsuaki Goh, Katsumi Higashi, Shoshi Muto, Kazuyuki Kuchitsu
JournalBiochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 317 Issue 3 Pg. 823-30 (May 7 2004) ISSN: 0006-291X [Print] United States
PMID15081414 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Algal Proteins
  • Calcium Channels
  • DNA Primers
  • Fungal Proteins
  • cryptogein protein, Phytophthora cryptogea
  • Calcium
  • Algal Proteins (pharmacology)
  • Amino Acid Sequence
  • Base Sequence
  • Calcium (physiology)
  • Calcium Channels (chemistry, drug effects, genetics, physiology)
  • Cell Line
  • Cloning, Molecular
  • DNA Primers
  • Fungal Proteins
  • Molecular Sequence Data
  • Saccharomyces cerevisiae (growth & development)
  • Sequence Homology, Amino Acid
  • Tobacco (cytology, physiology)

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