Abstract |
Yeast phase lysate antigens from 10 isolates of Blastomyces dermatitidis (dog, ERC-2 and T-58; T-27, polar bear; woodpile, ER-3; bat lung, 48938; human, B5929, B5895, B5896, B5931, and CAPP) from different geographical regions, in addition to a Histoplasma capsulatum (G217B) lysate preparation were compared with respect to their reactivity against serum specimens from dogs, rabbits and humans positive for blastomycosis using an indirect enzyme-linked immunosorbent assay. In addition, the lysate antigens were also assayed against histoplasmosis-positive human serum samples to study their cross-reactivity. Variable results were obtained with T-58 and T-27 exhibiting the greatest reactivity. We also noticed that the lysate did not react consistently to serum samples across species with lesser reactivity evidenced when testing dog sera. Finally, T-58 gave the highest cross-reactivity with histoplasmosis-positive sera. The study may prove valuable in the development of antigen candidates for blastomycosis serodiagnosis.
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Authors | R O Abuodeh, E M Chester, G M Scalarone |
Journal | Mycoses
(Mycoses)
Vol. 47
Issue 3-4
Pg. 143-9
(Apr 2004)
ISSN: 0933-7407 [Print] Germany |
PMID | 15078431
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Fungal
- Antigens, Fungal
- Immune Sera
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Topics |
- Animals
- Antibodies, Fungal
(blood)
- Antigens, Fungal
(immunology)
- Blastomyces
(immunology)
- Blastomycosis
(diagnosis, immunology, microbiology, veterinary)
- Cross Reactions
- Dog Diseases
(diagnosis, immunology, microbiology)
- Dogs
- Enzyme-Linked Immunosorbent Assay
- Humans
- Immune Sera
- Rabbits
- Ursidae
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