HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Activation of neutrophils and inhibition of the proinflammatory cytokine response by endogenous granulocyte colony-stimulating factor in murine pneumococcal pneumonia.

Abstract
Granulocyte colony-stimulating factor (G-CSF) is considered to improve host defense during infection, via increased recruitment of and enhanced performance of neutrophils and subsequent inhibition of potentially harmful proinflammatory mediators. The present study sought to determine the role of endogenous G-CSF in host defense against pneumococcal pneumonia. Patients with unilateral community-acquired pneumonia demonstrated elevated concentrations of G-CSF in bronchoalveolar lavage fluid obtained from the infected, but not from the contralateral, site. Treatment of mice with pneumococcal pneumonia with an anti-G-CSF antibody reduced neutrophil counts in lung tissue and diminished CD11b expression on pulmonary neutrophils but increased the lung concentrations of tumor necrosis factor- alpha, interleukin-1 beta, and cytokine-induced neutrophil chemoattractant. Treatment with anti-G-CSF did not influence the outgrowth of pneumococci in lungs, the dissemination of the infection, or survival in murine pneumonia. During pneumococcal pneumonia, G-CSF is produced locally at the site of the infection, where it exerts both pro- and anti-inflammatory effects.
AuthorsSylvia Knapp, Lars Hareng, Anita W Rijneveld, Paul Bresser, Jaring S van der Zee, Sandrine Florquin, Thomas Hartung, Tom van der Poll
JournalThe Journal of infectious diseases (J Infect Dis) Vol. 189 Issue 8 Pg. 1506-15 (Apr 15 2004) ISSN: 0022-1899 [Print] United States
PMID15073689 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • CD11b Antigen
  • Cytokines
  • Granulocyte Colony-Stimulating Factor
  • Peroxidase
Topics
  • Adult
  • Animals
  • Bronchoalveolar Lavage Fluid (immunology, microbiology)
  • CD11b Antigen (immunology)
  • Cell Count
  • Colony Count, Microbial
  • Community-Acquired Infections (immunology, microbiology, pathology)
  • Cytokines (immunology)
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Flow Cytometry
  • Granulocyte Colony-Stimulating Factor (immunology)
  • Humans
  • Lung (immunology, pathology)
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Neutrophil Activation (immunology)
  • Neutrophils (immunology, microbiology)
  • Peroxidase (immunology)
  • Pneumonia, Pneumococcal (immunology, microbiology, pathology)
  • Streptococcus pneumoniae (immunology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: