APC mutations are infrequent but present in human lung cancer.

Abstract	Recent studies have revealed the presence of beta-catenin mutations in a small subset of human and rat lung carcinomas, suggesting the involvement of the Wnt pathway in pulmonary carcinogenesis. LOH on chromosome 5q (APC locus) is frequent in lung cancer, but previous studies have found no adenomatous polyposis coli (APC) mutations. In this study, we screened 114 human lung cancer specimens for alterations in the mutation cluster region of the APC gene and in exon 3 of the beta-catenin gene. SSCP followed by direct DNA sequencing revealed APC mutations in 2/44 (5%) squamous cell carcinomas, a 2-bp deletion in codon 1465 (AGT-->A), and a GAA-->CAA (Glu-->Gln) mutation at codon 1317. One of 32 (3%) small cell lung carcinomas contained a GAA-->AAA (Glu-->Lys) mutation at codon 1284. Two cases with an APC mutation showed focal nuclear beta-catenin staining. These results suggest that disruption of the Wnt pathway through APC mutations is infrequent, but may be involved in the pathogenesis of a small subset of human lung carcinomas.
Authors	Hiroko Ohgaki, Johan M Kros, Yoshikazu Okamoto, Ariana Gaspert, Hervé Huang, Michael O Kurrer
Journal	Cancer letters (Cancer Lett) Vol. 207 Issue 2 Pg. 197-203 (Apr 30 2004) ISSN: 0304-3835 [Print] Ireland
PMID	15072829 (Publication Type: Journal Article)
Chemical References	CTNNB1 protein, human Cytoskeletal Proteins Proto-Oncogene Proteins Trans-Activators Wnt Proteins Zebrafish Proteins beta Catenin
Topics	Adenocarcinoma (genetics, metabolism, pathology) Adenomatous Polyposis Coli (genetics, metabolism, pathology) Adult Aged Aged, 80 and over Carcinoma, Small Cell (genetics, metabolism, pathology) Carcinoma, Squamous Cell (genetics, metabolism, pathology) Cytoskeletal Proteins (genetics, metabolism) Genes, APC (physiology) Humans Immunoenzyme Techniques Lung Neoplasms (genetics, metabolism, pathology) Middle Aged Mutation (genetics) Polymorphism, Single-Stranded Conformational Proto-Oncogene Proteins (genetics) Sequence Deletion Signal Transduction Trans-Activators (genetics, metabolism) Wnt Proteins Zebrafish Proteins beta Catenin

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.

Realize the full power of the drug-disease research graph!