Elevation of platelet-associated IgG in aplastic anemia.

Abstract

We determined platelet-associated IgG (PAIgG) levels in patients with aplastic anemia, idiopathic thrombocytopenic purpura (ITP), iron deficiency anemia, and systemic lupus erythematosus (SLE), as well as in normal healthy adults as a control group. To determine PAIgG levels, we used a competitive micro enzyme-linked immunosorbent assay, which had excellent reproducibility, recovery, and dilution. We confirmed its reliability by comparing it to the immunoradiometric assay. Both the aplastic anemia group (n = 27, mean +/- SD = 218.6 +/- 244.6 ng/10(7) platelets) and the ITP group (n = 82, mean +/- SD = 212.5 +/- 327.8 ng/10(7) platelets) had higher PAIgG levels than the SLE group (n = 4, mean +/- SD = 38.4 +/- 22.4 ng/10(7) platelets), iron deficiency anemia group (n = 10, mean +/- SD = 16.1 +/- 3.6 ng/10(7) platelets), and normal control group (n = 69, mean +/- SD = 16.1 +/- 3.6 ng/10(7) platelets. The higher platelet-associated IgG levels in aplastic anemia suggest that autoimmune mechanisms are involved.

• Authors: R Kawaguchi, S Haruna, K Hikiji, Y Higashi, Y Tsukada
• Journal: Journal of clinical laboratory analysis (J Clin Lab Anal) Vol. 6 Issue 3 Pg. 130-5 ( 1992) ISSN: 0887-8013 [Print] United States
• PMID: 1506979 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Immunoglobulin G

Topics

• Adolescent
• Adult
• Aged
• Anemia, Aplastic (immunology)
• Anemia, Hypochromic (immunology)
• Autoimmunity
• Blood Platelets (immunology)
• Child
• Child, Preschool
• Enzyme-Linked Immunosorbent Assay (methods, standards)
• Evaluation Studies as Topic
• Female
• Humans
• Immunoglobulin G (blood)
• Immunoradiometric Assay
• Infant
• Lupus Erythematosus, Systemic (immunology)
• Male
• Middle Aged
• Purpura, Thrombocytopenic, Idiopathic (immunology)
• Reference Standards