Abstract | PURPOSE: METHOD: The mRNA levels of TS, DPD, OPRT, TP, and UP were measured by real-time quantitative RT-PCR in samples from 23 consecutive patients with both primary colorectal adenocarcinoma and liver metastasis. RESULTS: The DPD, OPRT, TP, and UP mRNA levels were significantly higher in liver metastases than in primary tumor (expression in relation to that of beta-actin mRNA: 0.42 vs 0.16, P=0.00053; 1.4 vs 0.92, P=0.016; 23 vs 11, P=0.00014; 0.36 vs 0.25, P=0.0026; respectively). However, the TS mRNA level did not differ significantly between liver metastases than primary tumor (0.20 vs 0.16, P=0.28). No correlation was observed for any gene between primary tumor and liver metastases. In both primary tumor and liver metastasis, the TS mRNA levels correlated significantly with the OPRT mRNA level (primary rS=0.83, P=0.00000081; liver metastasis rS=0.49, P=0.017), while the DPD mRNA level correlated significantly with the TP mRNA level rS=0.81, P=0.0000024; rS=0.63, P=0.0014; respectively). CONCLUSIONS:
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Authors | Mikito Inokuchi, Hiroyuki Uetake, Yoshinori Shirota, Hiroyuki Yamada, Masayuki Tajima, Kenichi Sugihara |
Journal | Cancer chemotherapy and pharmacology
(Cancer Chemother Pharmacol)
Vol. 53
Issue 5
Pg. 391-6
(May 2004)
ISSN: 0344-5704 [Print] Germany |
PMID | 15060742
(Publication Type: Journal Article)
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Chemical References |
- Dihydrouracil Dehydrogenase (NADP)
- Orotate Phosphoribosyltransferase
- Uridine Phosphorylase
- Thymidine Phosphorylase
- Fluorouracil
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Topics |
- Colorectal Neoplasms
(drug therapy, enzymology)
- Dihydrouracil Dehydrogenase (NADP)
(genetics)
- Fluorouracil
(metabolism)
- Gene Expression Regulation, Enzymologic
- Humans
- Liver Neoplasms
(enzymology, secondary)
- Orotate Phosphoribosyltransferase
(genetics)
- Reverse Transcriptase Polymerase Chain Reaction
- Thymidine Phosphorylase
(genetics)
- Uridine Phosphorylase
(genetics)
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