Effect of a paraneoplastic cerebellar degeneration-associated neural protein on B-myb promoter activity.

Abstract

In this study, we have shown that a paraneoplastic cerebellar degeneration (PCD)-associated antigen, pcd17, binds to a cell cycle-related protein, MRG15. MRG15 derepresses the E2F-responsive B-myb promoter. The pcd17 antigen inhibits the derepression of the B-myb transcriptional activity by MRG15, and, as a result, pcd17 represses the promoter. Delivery of anti-Purkinje cell antibodies (anti-Yo) into the cells inhibits the repression of B-myb promoter activity by pcd17. Because derepression of the B-myb promoter has been implicated in neuronal death, the results suggest the possible role of the antibodies in the pathogenesis of PCD.

• Authors: Koichiro Sakai, Yoko Kitagawa, Shinji Saiki, Misuzu Saiki, Genjiro Hirose
• Journal: Neurobiology of disease (Neurobiol Dis) Vol. 15 Issue 3 Pg. 529-33 (Apr 2004) ISSN: 0969-9961 [Print] United States
• PMID: 15056460 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cell Cycle Proteins
• Chromosomal Proteins, Non-Histone
• DNA-Binding Proteins
• E2F Transcription Factors
• Immunoglobulin G
• Neoplasm Proteins
• Nerve Tissue Proteins
• Trans-Activators
• Transcription Factors

Topics

• Animals
• COS Cells
• Cell Cycle Proteins
• Chlorocebus aethiops
• Chromosomal Proteins, Non-Histone (drug effects, metabolism)
• DNA-Binding Proteins (drug effects, immunology, metabolism)
• E2F Transcription Factors
• Immunoglobulin G (pharmacology)
• Neoplasm Proteins (immunology)
• Nerve Degeneration (metabolism, pathology)
• Nerve Tissue Proteins (metabolism)
• Paraneoplastic Cerebellar Degeneration (physiopathology)
• Promoter Regions, Genetic
• Purkinje Cells (immunology, pathology)
• Trans-Activators (drug effects, metabolism)
• Transcription Factors (metabolism)
• Two-Hybrid System Techniques