Laboratory medicine. Series on clinical testing. 2. Fractionation of urinary ketosteroids. Procedure and clinical significance.

Abstract

Ketosteroids, the excretory metabolities of adrenal and gonadal steroids, can be analyzed individually in urine by a simple extraction procedure followed by separation and quantitation by the use of gas/liquid chromatography. The ketosteroids quantitatively detected by the technique are androsterone, etiocholanolone, dehydroepiandrosterone, 11-hydroxyandrosterone, 11-hydroxyetiocholanolone, 11-ketandrosterone, and 11-ketoetiocholanolone. Other steroid metabolities detected are pregnanediol, pregnanetriol, delta5-pregnenetriol, and 11-ketopregnanetriol. In comparison with concentrations of these steroids observed in urine specimens collected from healthy individuals, abnormal results occur in specimens from patients with testicular disease, Cushing's syndrome, adrenal hyperplasia, and several types of female hirsutism. Characteristic profiles for each of these diseases are presented.

• Authors: T P Moyer, P C Carpenter, N Jiang, D Machacek
• Journal: Mayo Clinic proceedings (Mayo Clin Proc) Vol. 53 Issue 9 Pg. 601-6 (Sep 1978) ISSN: 0025-6196 [Print] England
• PMID: 150475 (Publication Type: Journal Article)

Chemical References

• 11-Hydroxycorticosteroids
• 17-Ketosteroids
• Ketosteroids
• Pregnenes
• Pregnanetriol
• Dehydroepiandrosterone
• Etiocholanolone
• Androsterone
• Pregnanediol
• 11-ketoetiocholanolone

Topics

• 11-Hydroxycorticosteroids (urine)
• 17-Ketosteroids (urine)
• Adrenal Gland Diseases (diagnosis)
• Androsterone (analogs & derivatives, urine)
• Chemical Fractionation
• Chromatography, Gas
• Dehydroepiandrosterone (urine)
• Etiocholanolone (analogs & derivatives, urine)
• Female
• Hirsutism (diagnosis)
• Humans
• Ketosteroids (urine)
• Male
• Pregnanediol (urine)
• Pregnanetriol (urine)
• Pregnenes (urine)