Abstract | PURPOSE: EXPERIMENTAL DESIGN: Using the technique of immunohistochemistry, we determined Id-1 and Id-2 expression in a panel of 67 human prostate biopsies. We also manipulated Id-1 and Id-2 expression in LNCaP and PC3 prostate cancer cell lines and determined the effects on invasion in vitro, matrix metalloproteinase secretion, and proliferation. RESULTS: Both Id-1 and Id-2 proteins were up-regulated during human prostate cancer progression in vivo and were overexpressed in highly aggressive prostate cancer cells. In vitro, constitutive expression of Id-1, and to a lesser extent Id-2, converted nonaggressive LNCaP prostate cancer cells into more proliferative and invasive cells and increased their secretion of matrix metalloproteinases. Conversely, the down-regulation of Id-2 expression in highly metastatic PC3 cells reduced their growth potential and invasiveness. CONCLUSIONS:
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Authors | Jean-Philippe Coppe, Yoko Itahana, Dan H Moore, James L Bennington, Pierre-Yves Desprez |
Journal | Clinical cancer research : an official journal of the American Association for Cancer Research
(Clin Cancer Res)
Vol. 10
Issue 6
Pg. 2044-51
(Mar 15 2004)
ISSN: 1078-0432 [Print] United States |
PMID | 15041724
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Biomarkers, Tumor
- DNA-Binding Proteins
- ID1 protein, human
- ID2 protein, human
- Inhibitor of Differentiation Protein 1
- Inhibitor of Differentiation Protein 2
- Repressor Proteins
- Transcription Factors
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Topics |
- Biomarkers, Tumor
(analysis, genetics)
- Cell Line, Tumor
- DNA-Binding Proteins
(analysis, genetics)
- Disease Progression
- Helix-Loop-Helix Motifs
- Humans
- Inhibitor of Differentiation Protein 1
- Inhibitor of Differentiation Protein 2
- Male
- Neoplasm Invasiveness
- Prostatic Neoplasms
(genetics, pathology)
- Repressor Proteins
(analysis, genetics)
- Transcription Factors
(analysis, genetics)
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