Abstract | PURPOSE: EXPERIMENTAL DESIGN: Using several human lung carcinoma cell lines (small and non-small carcinoma cells), we assayed for cell growth inhibition and apoptosis induction. We also assayed for p21 mRNA and protein expression by reverse transcription-PCR, real-time reverse transcription-PCR, and Western blot analysis. Nuclear protein binding activities to three response elements located in the p21 promoter [nuclear factor ( NF)-kappaB, Sp1, and NF- interleukin 6 ( IL6) CAAT/enhancer binding protein (C/EBP)] were measured by gel mobility shift assays. We used transient transfection assays with p21 promoter reporter gene constructs to determine the transcriptional regulation by PPARgamma ligands. Finally, by using p21 antisense oligonucleotides, we tested the link between PPARgamma activation and p21 signaling in cell growth inhibition assays and by Western blot analysis. RESULTS: CONCLUSION:
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Authors | Shouwei Han, Neil Sidell, Paul B Fisher, Jesse Roman |
Journal | Clinical cancer research : an official journal of the American Association for Cancer Research
(Clin Cancer Res)
Vol. 10
Issue 6
Pg. 1911-9
(Mar 15 2004)
ISSN: 1078-0432 [Print] United States |
PMID | 15041706
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- DNA Primers
- Hypoglycemic Agents
- Ligands
- NF-kappa B
- Oligodeoxyribonucleotides, Antisense
- PPAR gamma
- Thiazolidinediones
- Transcription Factors
- Oncogene Protein p21(ras)
- ciglitazone
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Topics |
- Apoptosis
(drug effects)
- Base Sequence
- Cell Division
- Cell Line, Tumor
- DNA Primers
- Gene Expression Regulation, Neoplastic
(genetics)
- Humans
- Hypoglycemic Agents
(pharmacology)
- Ligands
- Lung Neoplasms
(genetics, pathology)
- NF-kappa B
(metabolism)
- Oligodeoxyribonucleotides, Antisense
(pharmacology)
- Oncogene Protein p21(ras)
(genetics)
- PPAR gamma
(physiology)
- Polymerase Chain Reaction
- Signal Transduction
(drug effects)
- Thiazolidinediones
(pharmacology)
- Transcription Factors
(metabolism)
- Transfection
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