[Effect of erythromycin on the expression of apoptotic gene on eosinophils in nasal polyps in vitro].

Abstract

OBJECTIVE:

To study the expression of apoptotic gene in nasal polyps and the possible effect of erythromycin on it.

METHODS:

Total 40 nasal polyps biopsies were detected the expression of Bcl-2, Bax, Bcl-xL as well as Fas protein on eosinophils by immunohistochemistry. The expression of Bax, Bcl-xL, Fas on eosinophils in 15 nasal polyps explants which cultured with or without erythromycin were detected also.

RESULTS:

In nasal polyps, the expression of Bcl-2, Bax, Bcl-xL and Fas protein on eosinophils are 5% (2/10), 65% (26/40), 40% (16/40) and 90% (36/40) respectively. As compared with inferior turbinate, there were significant difference in expression of Bax and Fas protein between two tissues (P < 0.05). After cultured with erythromycin, the expression of Bax protein increased (P < 0.05), but the expression of Bcl-xL and Fas protein had not changed.

CONCLUSION:

Bax may be the most significant gene which control the apoptosis of eosinophils in nasal polyps. And a possible mechaism which erythromycin promote the apoptosis of eosinophils is increase the expression of Bax.

• Authors: Jun Tang, Yuan Li
• Journal: Zhonghua er bi yan hou ke za zhi (Zhonghua Er Bi Yan Hou Ke Za Zhi) Vol. 38 Issue 6 Pg. 451-4 (Dec 2003) ISSN: 0412-3948 [Print] China
• PMID: 15040110 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• BAX protein, human
• Proto-Oncogene Proteins
• Proto-Oncogene Proteins c-bcl-2
• bcl-2-Associated X Protein
• fas Receptor
• Erythromycin

Topics

• Adolescent
• Adult
• Apoptosis (drug effects)
• Cells, Cultured
• Child
• Eosinophils (metabolism, pathology)
• Erythromycin (pharmacology)
• Female
• Gene Expression Regulation
• Humans
• Male
• Middle Aged
• Nasal Polyps (pathology)
• Proto-Oncogene Proteins (biosynthesis, genetics)
• Proto-Oncogene Proteins c-bcl-2 (biosynthesis, genetics)
• bcl-2-Associated X Protein
• fas Receptor (biosynthesis, genetics)