HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Purification, cloning and biological characterization of a novel disintegrin from Trimeresurus jerdonii venom.

Abstract
A novel disintegrin, jerdonin, was purified from the Trimeresurus jerdonii venom by means of gel filtration and reverse phase high pressure liquid chromatography. Its coding cDNA was also isolated from the venom gland. The jerdonin coding cDNA is part of a precursor composed of proprotein, metalloproteinase, and disintegrin domains. From the deduced amino acid sequence, jerdonin is composed of 71 amino acid residues including 12 cysteines and the tripeptide sequence Arg-Gly-Asp (RGD), a well-known characteristic of the disintegrin family. Molecular mass of jerdonin was determined to be 7483Da by matrix-assisted laser desorption ionization time of flight mass spectrometry. Jerdonin inhibited ADP- and collagen-induced human platelet aggregation with IC(50) of 220 and 240 nM, respectively. In vivo, jerdonin inhibited the growth of subcutaneously inoculated B16 solid tumor in C57BL/6 mice and improved the survival time of the tumor-bearing mice.
AuthorsXing-Ding Zhou, Yang Jin, Run-Qiang Chen, Qiu-Min Lu, Jian-Bo Wu, Wan-Yu Wang, Yu-Liang Xiong
JournalToxicon : official journal of the International Society on Toxinology (Toxicon) Vol. 43 Issue 1 Pg. 69-75 (Jan 2004) ISSN: 0041-0101 [Print] England
PMID15037031 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antineoplastic Agents
  • Crotalid Venoms
  • DNA, Complementary
  • Disintegrins
  • Oligopeptides
  • Trimeresurus venoms
  • jerdonin
  • arginyl-glycyl-aspartic acid
Topics
  • Amino Acid Sequence
  • Animals
  • Antineoplastic Agents (isolation & purification, pharmacology)
  • Base Sequence
  • Chromatography, High Pressure Liquid
  • Cloning, Molecular
  • Crotalid Venoms (chemistry, genetics, isolation & purification, pharmacology)
  • DNA, Complementary (genetics)
  • Disintegrins (chemistry, genetics, isolation & purification, pharmacology)
  • Melanoma, Experimental (drug therapy)
  • Mice
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Oligopeptides (genetics, isolation & purification, pharmacology)
  • Sequence Homology
  • Survival Rate
  • Trimeresurus

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: