Abstract |
Nontypeable Haemophilus influenzae (NTHi) is a leading etiologic agent of otitis media in children and recurrent respiratory infections in patients with chronic obstructive pulmonary disease. The highly conserved outer membrane protein P6 constitutes a promising vaccine candidate antigen. However, the small amount of P6 produced by this fastidious microorganism renders large-scale production difficult. Controversial data also exist concerning the suitability of recombinant P6 (rP6) as a vaccine antigen. Therefore, we performed a comparative evaluation of the immunogenicity and efficacy of native P6 and rP6 in mice intranasally vaccinated with adamantylamide dipeptide (AdDP) as an adjuvant. High titers of P6-specific serum antibodies were elicited in mice vaccinated with either native P6 or rP6, which cross-recognized both antigens. However, rP6 stimulated stronger mucosal responses. Mice vaccinated with rP6 were protected against both pulmonary and middle-ear infections (P<.01). This demonstrates that rP6 plus AdDP constitutes a promising vaccine formulation against the most relevant forms of disease caused by NTHi.
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Authors | Gustavo M Bertot, Pablo D Becker, Carlos A Guzmán, Saúl Grinstein |
Journal | The Journal of infectious diseases
(J Infect Dis)
Vol. 189
Issue 7
Pg. 1304-12
(Apr 01 2004)
ISSN: 0022-1899 [Print] United States |
PMID | 15031801
(Publication Type: Comparative Study, Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Adjuvants, Immunologic
- Antibodies, Bacterial
- Bacterial Outer Membrane Proteins
- Dipeptides
- Haemophilus Vaccines
- P6 outer membrane protein, Haemophilus
- Recombinant Proteins
- adamantylamide-alanyl-isoglutamine
- Amantadine
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Topics |
- Adjuvants, Immunologic
(administration & dosage)
- Administration, Intranasal
- Amantadine
(administration & dosage, analogs & derivatives)
- Animals
- Antibodies, Bacterial
(blood)
- Bacterial Outer Membrane Proteins
(immunology)
- Bronchoalveolar Lavage Fluid
(microbiology)
- Dipeptides
(administration & dosage)
- Female
- Haemophilus Infections
(immunology, microbiology, prevention & control)
- Haemophilus Vaccines
(administration & dosage, immunology)
- Haemophilus influenzae
(immunology)
- Immunization
(methods)
- Lung Diseases
(immunology, microbiology, prevention & control)
- Mice
- Mice, Inbred BALB C
- Otitis Media
(immunology, microbiology, prevention & control)
- Recombinant Proteins
(immunology)
- Saliva
(microbiology)
- Vagina
(microbiology)
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