Abstract | AIMS: METHODS: RESULTS: It is shown that dopamine and noradrenaline levels in the distal colon (inflamed mucosa), but not in the ileum (non-inflamed mucosa), of TNBS-treated rats were markedly lower than in control animals. A slight decrease in L-DOPA tissue levels, no changes in AADC activity and an increase in plasma IFN-gamma levels accompanied this decrease in dopamine levels. Exposure of Caco-2 cells, a human intestinal epithelial cell line, to human IFN-gamma resulted in a concentration-dependent and long-lasting inhibition of L-DOPA uptake, which most likely explains the decrease in dopamine levels in the inflamed mucosa. CONCLUSION:
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Authors | F Magro, S Fraga, T Ribeiro, P Soares-da-Silva |
Journal | Acta physiologica Scandinavica
(Acta Physiol Scand)
Vol. 180
Issue 4
Pg. 379-86
(Apr 2004)
ISSN: 0001-6772 [Print] England |
PMID | 15030379
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Levodopa
- Interferon-gamma
- Trinitrobenzenesulfonic Acid
- Aromatic-L-Amino-Acid Decarboxylases
- Dopamine
- Norepinephrine
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Topics |
- Animals
- Aromatic-L-Amino-Acid Decarboxylases
(metabolism)
- Caco-2 Cells
- Chromatography, High Pressure Liquid
(methods)
- Colitis
(metabolism)
- Colon
(metabolism)
- Dopamine
(analysis)
- Humans
- Ileum
(metabolism)
- Interferon-gamma
(blood, metabolism)
- Intestinal Mucosa
(metabolism)
- Levodopa
(pharmacokinetics)
- Male
- Norepinephrine
(analysis)
- Rats
- Rats, Wistar
- Trinitrobenzenesulfonic Acid
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