Abstract |
The effect of monoterpene perillyl alcohol (POH) on cell growth, cell cycle progression, and expression of cell cycle-regulatory proteins in estrogen receptor (ER)-positive (KPL-1 and MCF-7) and ER-negative (MKL-F and MDA-MB-231) human breast cancer cell lines was examined. POH inhibited cell proliferation in a dose-dependent manner in all cell lines tested. POH at a dose of 500 micro M had a cytostatic effect, in which growth inhibition was due to accumulation of cells in G1-phase. Cell cycle progression was preceded by a decrease in G1 cyclins ( cyclin D1 and E), followed by an increase in p21(Cip1/Waf1) and a decrease in proliferating cell nuclear antigen level. Levels of p53 and cyclin A were unchanged. POH at a dose of 75 mg/kg administered intraperitoneally three times a week throughout the entire 6-week experimental period suppressed orthotopically transplanted KPL-1 tumor cell growth and regional lymph node metastasis in a nude mouse system. POH inhibited both ER-positive and -negative human breast cancer cell growth in vitro, and suppressed growth and metastasis in vivo.
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Authors | Takashi Yuri, Naoyuki Danbara, Miki Tsujita-Kyutoku, Yasuhiko Kiyozuka, Hideto Senzaki, Nobuaki Shikata, Hideharu Kanzaki, Airo Tsubura |
Journal | Breast cancer research and treatment
(Breast Cancer Res Treat)
Vol. 84
Issue 3
Pg. 251-60
(Apr 2004)
ISSN: 0167-6806 [Print] Netherlands |
PMID | 15026623
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antineoplastic Agents
- Cyclins
- Monoterpenes
- Proliferating Cell Nuclear Antigen
- Receptors, Estrogen
- perillyl alcohol
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Topics |
- Animals
- Antineoplastic Agents
(pharmacology)
- Blotting, Western
- Breast Neoplasms
(drug therapy, pathology)
- Cell Cycle
(drug effects)
- Cyclins
(biosynthesis)
- Dose-Response Relationship, Drug
- Female
- Humans
- Immunohistochemistry
- Infusions, Parenteral
- Mice
- Mice, Nude
- Monoterpenes
(pharmacology)
- Neoplasm Metastasis
- Proliferating Cell Nuclear Antigen
(biosynthesis)
- Receptors, Estrogen
- Tumor Cells, Cultured
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