Abstract |
We assessed the usefulness of a mouse monoclonal antibody (13B10) against human herpesvirus 8 (HHV-8) latent nuclear antigen-1 (LNA-1) in diagnosis of Kaposi sarcoma (KS) and for distinguishing it from various mimickers by studying 50 cases of KS and 53 mimickers ( angiosarcoma, 15; kaposiform hemangioendothelioma, 6; spindle cell hemangioma, 3; reactive angioendotheliomatosis, 3; bacillary angiomatosis, 4; acroangiomatous dematitis, 2; microvenular hemangioma, 2; hobnail hemangioma, 2; pyogenic granuloma, 5; dermatofibroma, 8; arteriovenous hemangioma, 1; verrucous hemangioma, 1; nonspecific vascular proliferation, 1) from patients with or without acquired HIV infection. Immunohistochemical staining was performed on formalin-fixed, paraffin-embedded tissue sections. All 50 cases of KS were positive for HHV-8 LNA-1, with immunolocalization in the nuclei of the spindle cells and cells lining the primitive and thin-walled vascular channels, whereas all 53 mimickers (including 4 lesions from HIV-positive patients) tested negative. The results idicate that positive immunostaining for HHV-8 LNA- 1 exhibits high sensitivity and specificity for the diagnosis of KS and is, thus, useful for distinguishing it from the mimickers.
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Authors | Wah Cheuk, Kathy O Y Wong, Cesar S C Wong, J E Dinkel, David Ben-Dor, John K C Chan |
Journal | American journal of clinical pathology
(Am J Clin Pathol)
Vol. 121
Issue 3
Pg. 335-42
(Mar 2004)
ISSN: 0002-9173 [Print] England |
PMID | 15023037
(Publication Type: Journal Article)
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Chemical References |
- Antibodies, Monoclonal
- Nuclear Proteins
- Phosphoproteins
- latent nuclear antigen (LNA)
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Topics |
- Adult
- Antibodies, Monoclonal
- Diagnosis, Differential
- Herpesvirus 8, Human
(isolation & purification)
- Humans
- Immunohistochemistry
- Male
- Nuclear Proteins
(biosynthesis, immunology)
- Phosphoproteins
(biosynthesis, immunology)
- Sarcoma, Kaposi
(diagnosis, virology)
- Sensitivity and Specificity
- Skin Diseases
(diagnosis)
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