Antiendotoxin activity of protegrin analog IB-367 alone or in combination with piperacillin in different animal models of septic shock.

Abstract

The therapeutic efficacy of protegrin peptide IB-367 was investigated in three rat models of septic shock: (i) rats injected intraperitoneally with 1mg Escherichia coli 0111:B4 lipopolysaccharide, (ii) rats given an intraperitoneal injection of 2 X 10(10) CFU of E. coli ATCC 25922, and (iii) rats in which intra-abdominal sepsis was induced via cecal ligation and puncture. All animals were randomized to receive parenterally isotonic sodium chloride solution, 1mg/kg of IB-367, 60mg/kg piperacillin and 1mg/kg of IB-367 plus 60mg/kg piperacillin. The peptide demonstrated lower level of antimicrobial activity than piperacillin, nevertheless it exhibited the dual properties of antimicrobial and antiendotoxin agent. Finally IB-367 and piperacillin association showed to be the most effective therapeutic approach.

• Authors: Andrea Giacometti, Oscar Cirioni, Roberto Ghiselli, Federico Mocchegiani, Claudio Viticchi, Fiorenza Orlando, Giuseppina D'Amato, Maria Simona Del Prete, Wojciech Kamysz, Jerzy łLukasiak, Vittorio Saba, Giorgio Scalise
• Journal: Peptides (Peptides) Vol. 24 Issue 11 Pg. 1747-52 (Nov 2003) ISSN: 0196-9781 [Print] United States
• PMID: 15019206 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antimicrobial Cationic Peptides
• Lipopolysaccharides
• Peptides
• Proteins
• antimicrobial peptide IB-367
• Piperacillin

Topics

• Animals
• Antimicrobial Cationic Peptides
• Cecum (surgery)
• Disease Models, Animal
• Drug Therapy, Combination
• Escherichia coli Infections (drug therapy)
• Ligation
• Lipopolysaccharides (administration & dosage, pharmacology)
• Male
• Microbial Sensitivity Tests
• Peptides
• Peritonitis (drug therapy, etiology, microbiology)
• Piperacillin (administration & dosage, therapeutic use)
• Proteins (administration & dosage, therapeutic use)
• Punctures
• Rats
• Rats, Wistar
• Shock, Septic (drug therapy, etiology, microbiology)