Apoptotic and anti-adhesion effect of ajoene, a garlic derived compound, on the murine melanoma B16F10 cells: possible role of caspase-3 and the alpha(4)beta(1) integrin.

Abstract

In this study we evaluated the hypothesis that the antitumor activity of ajoene could be associated with its apoptosis-inducing effect, and with its ability to block the expression of the alpha(4)beta(1) integrin, in the murine melanoma B16F10 cells. Ajoene induced a significant reduction in B16F10 viability (IC(50)=62 microM), in a dose-dependent manner. Flow cytometric analysis showed that the cytotoxic effect of this compound was associated with caspase-3 activation. Ajoene at 25 microM altered the alpha(4)beta(1) integrin expression on B16F10, and induced a significant reduction in the adhesion of these cells to an endothelial cell monolayer.

• Authors: Eliades Ledezma, Rafael Apitz-Castro, José Cardier
• Journal: Cancer letters (Cancer Lett) Vol. 206 Issue 1 Pg. 35-41 (Mar 31 2004) ISSN: 0304-3835 [Print] Ireland
• PMID: 15019157 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• Disulfides
• Integrin alpha4beta1
• Plant Extracts
• Sulfoxides
• ajoene
• Glutathione Reductase
• Casp3 protein, mouse
• Caspase 3
• Caspases

Topics

• Animals
• Antineoplastic Agents (pharmacology)
• Apoptosis (drug effects)
• Caspase 3
• Caspases (metabolism)
• Cell Adhesion (drug effects)
• Colony-Forming Units Assay
• Disulfides (pharmacology)
• Flow Cytometry
• Garlic (chemistry)
• Glutathione Reductase (antagonists & inhibitors)
• Integrin alpha4beta1 (metabolism)
• Melanoma, Experimental (metabolism, pathology)
• Mice
• Plant Extracts (pharmacology)
• Sulfoxides
• Tumor Cells, Cultured