Microarray analysis of brain RNA in mice with methylenetetrahydrofolate reductase deficiency and hyperhomocysteinemia.

Abstract

Methylenetetrahydrofolate reductase (MTHFR) deficiency is the most common genetic cause of hyperhomocysteinemia, which is associated with increased risk for cardiovascular disease, stroke and possibly other neurological disorders. Microarray analysis of brain RNA from day 14 Mthfr(-/-) mice revealed several genes with altered expression. Expression changes in inositol 1,4,5-triphosphate receptor, type 1 (Itpr1), proteolipid protein (Plp), neurogenic differentiation factor 1 (Neurod1), S100 calcium binding protein A8 (S100a8), and methylenetetrahydrofolate dehydrogenase (NAD+ dependent), methenyltetrahydrofolate cyclohydrolase (Mthfd2) were confirmed by RT-PCR. We propose that neuronal damage by hyperhomocysteinemia may involve disruption of intracellular calcium.

• Authors: Zhoutao Chen, Bing Ge, Thomas J Hudson, Rima Rozen
• Journal: Brain research. Gene expression patterns (Brain Res Gene Expr Patterns) Vol. 1 Issue 2 Pg. 89-93 (Jan 2002) Netherlands
• PMID: 15018804 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Actins
• Basic Helix-Loop-Helix Transcription Factors
• Calcium Channels
• Calgranulin A
• Inositol 1,4,5-Trisphosphate Receptors
• Myelin Proteolipid Protein
• Nerve Tissue Proteins
• Receptors, Cytoplasmic and Nuclear
• Receptors, N-Methyl-D-Aspartate
• Transcription Factors
• Neurog1 protein, mouse
• RNA
• Methylenetetrahydrofolate Reductase (NADPH2)
• Methylenetetrahydrofolate Dehydrogenase (NADP)
• Methenyltetrahydrofolate Cyclohydrolase
• Calcium

Topics

• Actins (metabolism)
• Animals
• Basic Helix-Loop-Helix Transcription Factors
• Brain (metabolism)
• Calcium (metabolism)
• Calcium Channels (biosynthesis)
• Calgranulin A (biosynthesis)
• Expressed Sequence Tags
• Heterozygote
• Hyperhomocysteinemia (metabolism)
• Inositol 1,4,5-Trisphosphate Receptors
• Methenyltetrahydrofolate Cyclohydrolase (biosynthesis)
• Methylation
• Methylenetetrahydrofolate Dehydrogenase (NADP) (biosynthesis)
• Methylenetetrahydrofolate Reductase (NADPH2) (metabolism)
• Mice
• Mice, Inbred BALB C
• Myelin Proteolipid Protein (biosynthesis)
• Nerve Tissue Proteins (biosynthesis)
• Neurons (metabolism)
• Oligonucleotide Array Sequence Analysis
• RNA (metabolism)
• Receptors, Cytoplasmic and Nuclear (biosynthesis)
• Receptors, N-Methyl-D-Aspartate (metabolism)
• Reverse Transcriptase Polymerase Chain Reaction
• Time Factors
• Transcription Factors (biosynthesis)