Enhanced resistance to herpes simplex virus type 1 infection in transgenic mice expressing a soluble form of herpesvirus entry mediator.

Abstract

Herpesvirus entry mediator (HVEM) is a member of the tumor necrosis factor (TNF) receptor family used as a cellular receptor by virion glycoprotein D (gD) of herpes simplex virus (HSV). Both human and mouse forms of HVEM can mediate entry of HSV-1 but have no entry activity for pseudorabies virus (PRV). To assess the antiviral potential of HVEM in vivo, three transgenic mouse lines expressing a soluble form of HVEM (HVEMIg) consisting of an extracellular domain of murine HVEM and the Fc portion of human IgG1 were generated. All of the transgenic mouse lines showed marked resistance to HSV-1 infection when the mice were challenged intraperitoneally with HSV-1, but not to PRV infection. The present results demonstrate that HVEMIg is able to exert a significant antiviral effect against HSV-1 infection in vivo.

• Authors: Etsuro Ono, Saori Yoshino, Keiko Amagai, Satoshi Taharaguchi, Chiemi Kimura, Junko Morimoto, Manabu Inobe, Tomoko Uenishi, Toshimitsu Uede
• Journal: Virology (Virology) Vol. 320 Issue 2 Pg. 267-75 (Mar 15 2004) ISSN: 0042-6822 [Print] United States
• PMID: 15016549 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Receptors, Tumor Necrosis Factor
• Receptors, Tumor Necrosis Factor, Member 14
• Receptors, Virus
• TNFRSF14 protein, human
• Tnfrsf14 protein, mouse

Topics

• Animals
• Cells, Cultured
• Chlorocebus aethiops
• Fibroblasts
• Herpes Simplex (immunology, physiopathology, virology)
• Herpesvirus 1, Human (pathogenicity)
• Humans
• Mice
• Mice, Inbred C57BL (embryology)
• Mice, Transgenic
• Receptors, Tumor Necrosis Factor (genetics, metabolism)
• Receptors, Tumor Necrosis Factor, Member 14
• Receptors, Virus (genetics, metabolism)
• Vero Cells