Effect of oxybenzone on PGE2-production in vitro and on plaque and gingivitis in vivo.

Abstract

OBJECTIVES:

To study the effect of oxybenzone on prostaglandin E2 (PGE2) production in cell culture and to evaluate the effect of an oxybenzone-containing dentifrice on plaque and gingivitis in a 6-week clinical trial.

MATERIAL AND METHODS:

Human embryo palatal mesenchyme (HEPM) cells were used for testing the inhibition of IL-1beta-stimulated PGE2-production in vitro by different concentrations of oxybenzone. For the in vivo study, a total of 66 individuals with a Quigley & Hein plaque index of at least 1.5 and an Ainamo & Bay gingival index of at least 0.2 were included in a double-blind clinical trial with two cells and a parallel design. Two compositions of fluoride dentifrice were used, one with the addition of 0.5% oxybenzone, and one without. Plaque and gingival index were obtained at three time points: (1) at baseline, (2) after 3 weeks, and (3) after 6 weeks.

RESULTS:

A dose-dependent inhibition of PGE2-production was found in the HEPM cell culture following oxybenzone exposure. In the clinical trial, a 25% reduction of gingival index was observed in the oxybenzone group (p<0.001) after 6 weeks as compared with 2% for the placebo group.

CONCLUSIONS:

These findings indicate that PGE2-production is reduced by oxybenzone in vitro and that the use of oxybenzone in a dentifrice reduces gingivitis in vivo.

• Authors: Lillemor Jannesson, Dowen Birkhed, Dale Scherl, Abdul Gaffar, Stefan Renvert
• Journal: Journal of clinical periodontology (J Clin Periodontol) Vol. 31 Issue 2 Pg. 91-4 (Feb 2004) ISSN: 0303-6979 [Print] United States
• PMID: 15016032 (Publication Type: Clinical Trial, Comparative Study, Controlled Clinical Trial, Journal Article)
• Copyright: Copyright Blackwell Munksgaard, 2004.

Chemical References

• Benzophenones
• Dentifrices
• Interleukin-1
• Placebos
• oxybenzone
• Dinoprostone

Topics

• Adolescent
• Adult
• Aged
• Benzophenones (pharmacology, therapeutic use)
• Cell Culture Techniques
• Dental Plaque (prevention & control)
• Dental Plaque Index
• Dentifrices (therapeutic use)
• Dinoprostone (antagonists & inhibitors)
• Dose-Response Relationship, Drug
• Double-Blind Method
• Follow-Up Studies
• Gingivitis (prevention & control)
• Humans
• Interleukin-1 (pharmacology)
• Mesoderm (drug effects)
• Middle Aged
• Periodontal Index
• Placebos