Abstract |
Mitogen-activated protein kinase kinase 2 (MEK2), the upstream kinase of extracellular signal-regulated kinase 1/2 (ERK1/2) was previously isolated as cancer cell dissociation related factor. In this study, to further clarify the regulatory mechanism of cancer cell dissociation, two hamster (PC-1.0 and PC-1) and human (Capan-2 and AsPC-1) pancreatic cancer cell lines were analyzed immunocytochemically with anti-ERK1, anti-ERK2 and anti-phosphorylated ERK1/2 (p-ERK1/2) antibodies. U0126 (a MEK1/2 inhibitor) significantly suppressed ERK2 and p-ERK1/2 expressions in PC-1.0 and AsPC-1 cells (P<0.05). Cancer cell dissociation factor (DF) markedly induced ERK2 and p-ERK1/2 expressions in PC-1 and Capan-2 cells (P<0.05), and the induced ERK2 and p-ERK1/2 expressions were inhibited by subsequent U0126-treatment (P<0.05). Simultaneously, light microscopic images showed that DF clearly induced cell dissociation in PC-1 and Capan-2 cells, while U0126-treatment induced cell aggregation in these pancreatic cancer cells. ERK2 activation is closely involved in cell dissociation of pancreatic cancer cells.
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Authors | Xiaodong Tan, Hiroshi Egami, Shinji Ishikawa, Takashi Kurizaki, Yasuhiro Tamori, Eiji Takai, Masahiko Hirota, Michio Ogawa |
Journal | International journal of oncology
(Int J Oncol)
Vol. 24
Issue 4
Pg. 815-20
(Apr 2004)
ISSN: 1019-6439 [Print] Greece |
PMID | 15010817
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Butadienes
- Enzyme Inhibitors
- Nitriles
- U 0126
- Mitogen-Activated Protein Kinase 1
- Mitogen-Activated Protein Kinase 3
- Mitogen-Activated Protein Kinases
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Topics |
- Animals
- Butadienes
(pharmacology)
- Cell Aggregation
(drug effects)
- Cricetinae
- Enzyme Activation
(drug effects)
- Enzyme Inhibitors
(pharmacology)
- Fluorescent Antibody Technique
- Humans
- Mitogen-Activated Protein Kinase 1
(antagonists & inhibitors, metabolism)
- Mitogen-Activated Protein Kinase 3
- Mitogen-Activated Protein Kinases
(antagonists & inhibitors, metabolism)
- Nitriles
(pharmacology)
- Pancreatic Neoplasms
(enzymology, pathology)
- Phosphorylation
(drug effects)
- Signal Transduction
(drug effects)
- Tumor Cells, Cultured
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