Laminin-1 and SIKVAV a laminin-1-derived peptide, regulate the morphology and protease activity of a human salivary gland adenoid cystic carcinoma cell line.

Abstract

In a previous paper, we demonstrated that laminin-1 and its derived peptide SIKVAV modulates the morphology of an adenoid cystic carcinoma cell line (CAC2 cells). Light microscopy of CAC2 cells grown in three-dimensional preparations of SIKVAV-enriched laminin-1 showed the presence of pseudocystic spaces. Pseudocysts are hallmarks of adenoid cystic carcinoma in vivo. We hypothesized that these pseudocystic spaces could be due to the protease-inducing/activating role of SIKVAV. Thus, we studied the presence of matrix metalloproteinases (MMPs) in CAC2 cells treated either by laminin-1 or by SIKVAV-enriched laminin-1. Immunohistochemistry and zymography suggested that SIKVAV enhanced the secretion of MMP-2 and MMP-9 in CAC2 cells. We propose that SIKVAV induces pseudocystic formation probably through the secretion of MMPs 2 and 9.

• Authors: V M Freitas, B Scheremeta, M P Hoffman, R G Jaeger
• Journal: Oral oncology (Oral Oncol) Vol. 40 Issue 5 Pg. 483-9 (May 2004) ISSN: 1368-8375 [Print] England
• PMID: 15006619 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Culture Media
• Laminin
• Oligopeptides
• laminin 1
• seryl-isoleucyl-lysyl-valyl-alanyl-valinamide
• Matrix Metalloproteinases
• Matrix Metalloproteinase 2
• Matrix Metalloproteinase 9

Topics

• Carcinoma, Adenoid Cystic (enzymology, pathology)
• Cell Line, Tumor (drug effects)
• Culture Media
• Cytoplasm (chemistry)
• Extracellular Space (chemistry)
• Humans
• Immunohistochemistry (methods)
• Laminin (metabolism, pharmacology)
• Matrix Metalloproteinase 2 (analysis)
• Matrix Metalloproteinase 9 (analysis)
• Matrix Metalloproteinases (analysis)
• Oligopeptides (metabolism, pharmacology)
• Salivary Gland Neoplasms (enzymology, pathology)