Role of Syndecan-4 in the cellular invasion of Orientia tsutsugamushi.

Abstract	Cell surface heparan sulfate proteoglycans (HSPGs) play a critical role in the cellular invasion of intracellular bacteria and are presumed to have a role in the infection of host cells by Orientia tsutsugamushi. Previously, we showed that O. tsutsugamushi infection decreased markedly after treating host cells with heparinase, which suggests that HSPGs play an important role in oriential infection. We tested oriential infection in REF-Syn4 cells over-expressing syndecan-4, and in REF-Syn4AS cells in which the expression of syndecan-4 was down regulated by transfecting with anti-sense syndecan-4 cDNA. Oriential infection was found to be dependent on the expression level of syndecan-4 on the cell surface. Furthermore, the infectivity of O. tsutsugamushi was specifically reduced by treating O. tsutsugamushi with the purified recombinant core protein of syndecan-4 (Syn4E). These results suggest that the core protein of syndecan-4 and the heparin/heparan sulfate chain of syndecan play an important role in oriential infection by O. tsutsugamushi.
Authors	Hang-Rae Kim, Myung-Sik Choi, Ik-Sang Kim
Journal	Microbial pathogenesis (Microb Pathog) Vol. 36 Issue 4 Pg. 219-25 (Apr 2004) ISSN: 0882-4010 [Print] England
PMID	15001228 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	DNA, Antisense Heparan Sulfate Proteoglycans Membrane Glycoproteins Proteoglycans SDC4 protein, human Sdc4 protein, mouse Sdc4 protein, rat Syndecan-4 Heparin Heparin Lyase
Topics	Animals Cell Line Cell Membrane (chemistry, physiology) Cricetinae DNA, Antisense Fibroblasts (chemistry, metabolism, microbiology) Gene Expression Regulation Heparan Sulfate Proteoglycans (metabolism) Heparin (metabolism) Heparin Lyase (metabolism) Humans Membrane Glycoproteins (biosynthesis, chemistry, genetics, physiology) Mice Orientia tsutsugamushi (growth & development, metabolism, pathogenicity) Proteoglycans (biosynthesis, chemistry, genetics, physiology) Rats Syndecan-4

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