Effects of a beta3-adrenergic agonist on the immune response in diet-induced (cafeteria) obese animals.

Abstract

Molecules with affinity for beta3-adrenoceptors are not only effective anti-obesity agents in rodent models, but may play a role in the regulation of the immune response. The aim of the current investigation was to analyse the effects of trecadrine on the immune response in diet-induced (cafeteria) obese rats. Male Wistar rats were divided into 2 groups, the control group (C, n=9) was fed with the standard pelleted chow laboratory diet, while the other group was fed with a high-fat (cafeteria) diet. Cafeteria-fed rats were divided into two new subgroups (n=9 each), which received either i.p. saline (obese, O) or trecadrine (1mg/kg/day) (obese+trecadrine, O+T) daily for 5 weeks. Lymphocyte subpopulations and the proliferative response were determined by validated procedures. The administration of trecadrine was able to prevent the onset of obesity in cafeteria-fed rats. Trecadrine-treatment to obese animals appeared to improve the number of lymphocyte subpopulations (CD4+ and CD8+) as compared to those animals only receiving the high-fat diet, being the values of the trecadrine-treated animals on the high-fat diet similar to the control rats. However, the lymphoproliferative response when stimulated with several mitogens was markedly reduced by the cafeteria intake and was further decreased by the beta3-adrenergic administration. The spleen mRNA expression level of UCP2, PPARgamma and Ob-Rb were not affected by the trecadrine treatment. Summing up, at the immune system level, trecadrine administration increased the proportion of CD4+ spleen lymphocytes, although it was not able to restore the lymphocyte proliferative response which was depressed.

• Authors: O Lamas, J A Martínez, A Marti
• Journal: Journal of physiology and biochemistry (J Physiol Biochem) Vol. 59 Issue 3 Pg. 183-91 (Sep 2003) ISSN: 1138-7548 [Print] Spain
• PMID: 15000449 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Adrenergic beta-3 Receptor Agonists
• Adrenergic beta-Agonists
• Benzyl Alcohols
• Fatty Acids, Nonesterified
• Ion Channels
• Membrane Transport Proteins
• Mitochondrial Proteins
• RNA, Messenger
• Receptors, Cytoplasmic and Nuclear
• Transcription Factors
• Triglycerides
• Ucp2 protein, rat
• Uncoupling Protein 2
• trecadrine
• Cholesterol
• Glycerol

Topics

• Adipose Tissue (drug effects)
• Adrenergic beta-3 Receptor Agonists
• Adrenergic beta-Agonists (pharmacology)
• Animal Feed
• Animals
• Benzyl Alcohols (pharmacology)
• Body Weight (drug effects)
• Cholesterol (blood)
• Fatty Acids, Nonesterified (blood)
• Gene Expression (drug effects)
• Glycerol (blood)
• Ion Channels
• Male
• Membrane Transport Proteins (biosynthesis)
• Mitochondrial Proteins (biosynthesis)
• Obesity (blood, drug therapy, immunology)
• RNA, Messenger (biosynthesis)
• Rats
• Rats, Wistar
• Receptors, Cytoplasmic and Nuclear (biosynthesis)
• Spleen (cytology, metabolism)
• T-Lymphocytes, Cytotoxic (drug effects, immunology)
• T-Lymphocytes, Helper-Inducer (drug effects, immunology)
• Transcription Factors (biosynthesis)
• Triglycerides (blood)
• Uncoupling Protein 2