Abstract | AIMS: To investigate the in vitro antiviral properties of putranjivain A, isolated from the whole plant of Euphorbia jolkini Bioss (Euphorbiaceae). METHODS AND RESULTS: Herpes simplex virus (HSV)-2 strain 196-infected Vero cells were used. It was shown that putranjivain A exhibited antiviral activity with an IC50 of 7.9 +/- 1.2 micro M using the XTT assay, with the IC50 value increasing with increasing multiplicity of infection. Using the plaque reduction assay, the IC50 and IC90 were 6.3 +/- 0.8 and 14.5 +/- 3.1 micro M, respectively. Putranjivain A showed no cytotoxic effect on cell multiplication at concentrations that achieved antiviral activity. The 50% cell cytotoxic concentration (CC50) was 80.3 +/- 14.7 microM, and the selectivity index (SI) (ratio of CC50 to IC50) for the XTT and plaque reduction assays was 10.2 and 12.7, respectively. When tested for virucidal activity, putranjivain A significantly reduced viral infectivity at concentrations of 75 and 100 micro M, but not at 50 microM or below. The results of the time-of-addition studies suggested that putranjivain A affected the late stage of HSV-2 replication at 25 microM. Interestingly, putranjivain A also showed inhibition of viral attachment and cell penetration. The combination of putranjivain A and aciclovir produced no interaction. CONCLUSIONS:
Putranjivain A possesses antiviral activity, inhibiting viral attachment and penetration, and also interfering with the late stage of viral replication.
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Authors | Hua-Yew Cheng, Ta-Chen Lin, Chien-Min Yang, Kuo-Chih Wang, Liang-Tzung Lin, Chun-Ching Lin |
Journal | The Journal of antimicrobial chemotherapy
(J Antimicrob Chemother)
Vol. 53
Issue 4
Pg. 577-83
(Apr 2004)
ISSN: 0305-7453 [Print] England |
PMID | 14998984
(Publication Type: Journal Article)
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Chemical References |
- Antiviral Agents
- Glucosides
- Plant Extracts
- putranjivain A
- Gallic Acid
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Topics |
- Animals
- Antiviral Agents
(isolation & purification, pharmacology)
- Chlorocebus aethiops
- Dose-Response Relationship, Drug
- Euphorbia
- Gallic Acid
(analogs & derivatives, isolation & purification, pharmacology)
- Glucosides
(isolation & purification, pharmacology)
- Herpesvirus 2, Human
(drug effects, physiology)
- Plant Extracts
(isolation & purification, pharmacology)
- Plant Structures
- Vero Cells
- Virus Replication
(drug effects)
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