Abstract |
Treatment of severe arteriosclerosis obliterans of lower extremities (ASOLE) remains a clinical challenge. To develop a more effective approach, we evaluated the clinical efficacy of autologous transplantation of mobilized peripheral blood stem cells (PBSCs) in 5 patients with ASOLE. The patients received recombinant human granulocyte colony-stimulating factor (rhG-CSF, 600 micro g/day) for 5 consecutive days. On day 5, PBSCs were collected, sorted from blood circulation of patients, and then intramuscularly injected into their ischemic lower limbs. A significant improvement of clinical manifestations including severe pain, skin temperature and ulcer, was observed, without obvious adverse effect. The patient's limb was successfully saved. Satisfactory remission was obtained 3 months after transplantation as shown by significant improvement in ankle-brachial pressure index (ABI), blood flow in personal vascular laboratory (PVL), laser Doppler blood perfusion, and the angio-graphic scores. Our data suggest for the first time that autologous transplantation of mobilized PBSCs provides a practical, safe, and effective method of treatment for lower limb ischemia.
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Authors | Ping Ping Huang, Shan Zhu Li, Ming Zhe Han, Zhi Jian Xiao, Ren Chi Yang, Lu Gui Qiu, Zhong Chao Han |
Journal | Thrombosis and haemostasis
(Thromb Haemost)
Vol. 91
Issue 3
Pg. 606-9
(Mar 2004)
ISSN: 0340-6245 [Print] Germany |
PMID | 14983238
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Recombinant Proteins
- Granulocyte Colony-Stimulating Factor
- Granulocyte-Macrophage Colony-Stimulating Factor
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Topics |
- Aged
- Angiography
- Arteriosclerosis Obliterans
(therapy)
- Body Temperature
- Granulocyte Colony-Stimulating Factor
(metabolism)
- Granulocyte-Macrophage Colony-Stimulating Factor
(therapeutic use)
- Humans
- Laser-Doppler Flowmetry
- Lower Extremity
(pathology)
- Middle Aged
- Pain
- Perfusion
- Recombinant Proteins
(therapeutic use)
- Stem Cell Transplantation
(methods)
- Stem Cells
(cytology)
- Time Factors
- Transplantation, Autologous
(methods)
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