Pharmacokinetics, safety, and efficacy of a liposome encapsulated thymidylate synthase inhibitor, OSI-7904L [(S)-2-[5-[(1,2-dihydro-3-methyl-1-oxobenzo[f]quinazolin-9-yl)methyl]amino-1-oxo-2-isoindolynl]-glutaric acid] in mice.

Abstract

OSI-7904L [(S)-2-[5-[(1,2-dihydro-3-methyl-1-oxobenzo[f]quinazolin-9-yl)methyl]amino-1-oxo-2-isoindolynl]-glutaric acid] is a liposomal formulation of the highly specific, noncompetitive, thymidylate synthase inhibitor OSI-7904 (also known as GW1843, 1843U89, and GS7904). The liposome formulation was developed to enhance the therapeutic index and dose schedule convenience of this potent antifolate compound. The studies presented here were conducted to determine the antitumor efficacy, distribution, pharmacokinetics, and safety of OSI-7904L in mice. In a human colon adenocarcinoma xenograft model in mice, OSI-7904L demonstrated superior antitumor efficacy compared with OSI-7904 or 5-fluorouracil. Furthermore, OSI-7904L could be administered less frequently than OSI-7904 although still generating greater tumor growth inhibition. Distribution studies confirmed that OSI-7904L-treated animals had much greater plasma, tissue, and tumor exposure than did OSI-7904-treated animals. Tumor exposures, based on area under the curve, in OSI-7904L-treated mice were increased over 100-fold compared with tumor exposures in OSI-7904-treated mice. Plasma exposures following OSI-7904L administration were greater than dose proportional consistent with saturation of plasma clearance mechanisms. OSI-7904L was much more toxic than OSI-7904 in the mouse with primary toxicities to the intestines, bone marrow, and thymus. Minimal toxicity to the lungs and liver was noted. These data clearly demonstrated that in mice, OSI-7904L has an increased plasma residence time as well as increased tissue and tumor exposure compared with OSI-7904, thus resulting in increased potency and toxicity. Potential benefits of OSI-7904L include improved efficacy and a more convenient schedule of administration.

• Authors: John Desjardins, David L Emerson, Dorothy B Colagiovanni, Elizabeth Abbott, Eric N Brown, Daniel W Drolet
• Journal: The Journal of pharmacology and experimental therapeutics (J Pharmacol Exp Ther) Vol. 309 Issue 3 Pg. 894-902 (Jun 2004) ISSN: 0022-3565 [Print] United States
• PMID: 14982966 (Publication Type: Journal Article)

Chemical References

• Antineoplastic Agents
• Carbon Radioisotopes
• Drug Carriers
• Enzyme Inhibitors
• Indoles
• Isoindoles
• Liposomes
• Quinazolines
• 1843U89
• Thymidylate Synthase

Topics

• Animals
• Antineoplastic Agents (administration & dosage, adverse effects, blood, pharmacokinetics, therapeutic use)
• Biological Availability
• Carbon Radioisotopes
• Disease Models, Animal
• Drug Carriers
• Drug Delivery Systems
• Enzyme Inhibitors (administration & dosage, adverse effects, pharmacokinetics, therapeutic use)
• Humans
• Indoles (administration & dosage, adverse effects, blood, pharmacokinetics, therapeutic use)
• Isoindoles
• Liposomes
• Metabolic Clearance Rate
• Mice
• Mice, Inbred BALB C
• Neoplasm Transplantation
• Neoplasms, Experimental (drug therapy)
• Quinazolines (administration & dosage, adverse effects, blood, pharmacokinetics, therapeutic use)
• Thymidylate Synthase (antagonists & inhibitors)
• Tissue Distribution
• Treatment Outcome
• Tumor Cells, Cultured
• Xenograft Model Antitumor Assays